OppA

• Description: oligopeptide ABC transporter (binding protein)
  
  

Gene name	oppA
Synonyms	spo0KA
Essential	no
Product	oligopeptide ABC transporter (binding protein)
Function	initiation of sporulation, competence development
Gene expression levels in SubtiExpress: oppA
Interactions involving this protein in SubtInteract: OppA
MW, pI	61 kDa, 5.722
Gene length, protein length	1635 bp, 545 aa
Immediate neighbours	trpS, oppB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

ABC transporters, utilization of nitrogen sources other than amino acids, genetic competence, phosphorelay, membrane proteins

This gene is a member of the following regulons

ScoC regulon, TnrA regulon

The gene

Basic information

• Locus tag: BSU11430

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • required for the uptake of quorum sensing peptides such as PhrH PubMed

• Protein family: bacterial solute-binding protein 5 family (according to Swiss-Prot)

• Paralogous protein(s): DppE

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylation on (Tyr-301 OR Tyr-303) AND Thr-470 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • (OppD-OppF)-(OppB-OppC)-OppA PubMed

• Localization:
  • attached to the cell membrane (via OppB-OppC) PubMed
  • at the outer side of the cell: extracellular (signal peptide) PubMed, lipid modification as retention signal PubMed

Database entries

• Structure:

• UniProt: P24141

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: oppA-oppB-oppC-oppD-oppF PubMed

• Expression browser: oppA PubMed

• Sigma factor:

• Regulation:
  • expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
  • repressed during exponential growth (ScoC) PubMed

• Regulatory mechanism:
  • TnrA: transcription activation PubMed
  • ScoC: transcription repression PubMed

• Additional information:
  • A ncRNA is predicted between trpS and oppA PubMed

Biological materials

• Mutant: BP67 (spc) available in Stülke lab
  • 1S118 ( oppA::spec), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed