RelA

• Description: GTP pyrophosphokinase (stringent response)
  
  

Gene name	relA
Synonyms
Essential	no
Product	GTP pyrophosphokinase
Function	synthesis and degradation of (p)ppGpp, triggering the stringent response
Gene expression levels in SubtiExpress: relA
Metabolic function and regulation of this protein in SubtiPathways: Purine salvage, Nucleotides (regulation), Murein recycling
MW, pI	84 kDa, 6.964
Gene length, protein length	2202 bp, 734 aa
Immediate neighbours	yrvI, apt
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

metabolism of signalling nucleotides

The gene

Basic information

• Locus tag: BSU27600

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + GTP = AMP + guanosine 3'-diphosphate 5'-triphosphate (according to Swiss-Prot)

• Protein family: relA/spoT family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: O54408

• KEGG entry: [3]

• E.C. number: 2.7.6.5

Expression and regulation

• Operon: relA-yrvI PubMed

• Expression browser: relA PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Mohamed Marahiel, Marburg University, Germany homepage

References

Ezequiel Wexselblatt, Yaara Oppenheimer-Shaanan, Ilana Kaspy, Nir London, Ora Schueler-Furman, Eylon Yavin, Gad Glaser, Joshua Katzhendler, Sigal Ben-Yehuda
Relacin, a novel antibacterial agent targeting the Stringent Response.
PLoS Pathog: 2012, 8(9);e1002925
[PubMed:23028324] [WorldCat.org] [DOI] (I p)

Yousuke Natori, Kazumi Tagami, Kana Murakami, Sawako Yoshida, Osamu Tanigawa, Yoonsuh Moh, Kenta Masuda, Tetsuya Wada, Shota Suzuki, Hideaki Nanamiya, Yuzuru Tozawa, Fujio Kawamura
Transcription activity of individual rrn operons in Bacillus subtilis mutants deficient in (p)ppGpp synthetase genes, relA, yjbM, and ywaC.
J Bacteriol: 2009, 191(14);4555-61
[PubMed:19447912] [WorldCat.org] [DOI] (I p)

Shuyu Zhang, W G Haldenwang
RelA is a component of the nutritional stress activation pathway of the Bacillus subtilis transcription factor sigma B.
J Bacteriol: 2003, 185(19);5714-21
[PubMed:13129942] [WorldCat.org] [DOI] (P p)

Thomas M Wendrich, Gregor Blaha, Daniel N Wilson, Mohamed A Marahiel, Knud H Nierhaus
Dissection of the mechanism for the stringent factor RelA.
Mol Cell: 2002, 10(4);779-88
[PubMed:12419222] [WorldCat.org] [DOI] (P p)

Takashi Inaoka, Kosaku Takahashi, Mayumi Ohnishi-Kameyama, Mitsuru Yoshida, Kozo Ochi
Guanine nucleotides guanosine 5'-diphosphate 3'-diphosphate and GTP co-operatively regulate the production of an antibiotic bacilysin in Bacillus subtilis.
J Biol Chem: 2003, 278(4);2169-76
[PubMed:12372825] [WorldCat.org] [DOI] (P p)

Takashi Inaoka, Kozo Ochi
RelA protein is involved in induction of genetic competence in certain Bacillus subtilis strains by moderating the level of intracellular GTP.
J Bacteriol: 2002, 184(14);3923-30
[PubMed:12081964] [WorldCat.org] [DOI] (P p)

Christine Eymann, Georg Homuth, Christian Scharf, Michael Hecker
Bacillus subtilis functional genomics: global characterization of the stringent response by proteome and transcriptome analysis.
J Bacteriol: 2002, 184(9);2500-20
[PubMed:11948165] [WorldCat.org] [DOI] (P p)

S Autret, A Levine, F Vannier, Y Fujita, S J Séror
The replication checkpoint control in Bacillus subtilis: identification of a novel RTP-binding sequence essential for the replication fork arrest after induction of the stringent response.
Mol Microbiol: 1999, 31(6);1665-79
[PubMed:10209741] [WorldCat.org] [DOI] (P p)

T M Wendrich, M A Marahiel
Cloning and characterization of a relA/spoT homologue from Bacillus subtilis.
Mol Microbiol: 1997, 26(1);65-79
[PubMed:9383190] [WorldCat.org] [DOI] (P p)