RsfA

• Description: probable regulator of transcription of SigF-dependent genes
  
  

Gene name	rsfA
Synonyms	ywfN, ipa-92r
Essential	no
Product	regulator of SigF activity
Function	control of expression of SigF-dependent genes
Gene expression levels in SubtiExpress: rsfA
MW, pI	29 kDa, 5.13
Gene length, protein length	774 bp, 258 aa
Immediate neighbours	ywzC, ywfM
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

sigma factors and their control, sporulation proteins

This gene is a member of the following regulons

RsfA regulon, SigF regulon, SigG regulon

The RsfA regulon:

• katX, rsfA

The gene

Basic information

• Locus tag: BSU37620

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • forespore (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P39650

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: rsfA PubMed

• Expression browser: rsfA PubMed

• Sigma factor: SigF PubMed, SigG PubMed

• Regulation:
  • expressed during sporulation in the forespore (SigF) PubMed
  • repressed by casamino acids PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • 1S132 ( rsfA::tet), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Stephanie T Wang, Barbara Setlow, Erin M Conlon, Jessica L Lyon, Daisuke Imamura, Tsutomu Sato, Peter Setlow, Richard Losick, Patrick Eichenberger
The forespore line of gene expression in Bacillus subtilis.
J Mol Biol: 2006, 358(1);16-37
[PubMed:16497325] [WorldCat.org] [DOI] (P p)

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J Bacteriol: 2002, 184(15);4288-95
[PubMed:12107147] [WorldCat.org] [DOI] (P p)

L J Wu, J Errington
Identification and characterization of a new prespore-specific regulatory gene, rsfA, of Bacillus subtilis.
J Bacteriol: 2000, 182(2);418-24
[PubMed:10629188] [WorldCat.org] [DOI] (P p)

P Glaser, F Kunst, M Arnaud, M P Coudart, W Gonzales, M F Hullo, M Ionescu, B Lubochinsky, L Marcelino, I Moszer
Bacillus subtilis genome project: cloning and sequencing of the 97 kb region from 325 degrees to 333 degrees.
Mol Microbiol: 1993, 10(2);371-84
[PubMed:7934828] [WorldCat.org] (P p)