TagT

• Description: phosphotransferase, attaches teichoic acid, teichuronic acid, or acidic capsular polysaccharide to cell wall peptidoglycan via phosphodiester linkage to N-acteylmuramic acid
  
  

Gene name	ywtF
Synonyms
Essential	no
Product	phosphotransferase, responsible for attachment of anionic polymers to peptidoglycan
Function	transfer of anionic cell wall polymers from lipid-linked precursors to peptidoglycan
Gene expression levels in SubtiExpress: tagT
Interactions involving this protein in SubtInteract: TagT
MW, pI	35 kDa, 9.554
Gene length, protein length	966 bp, 322 aa
Immediate neighbours	ywtG, ywtE
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall synthesis, cell envelope stress proteins (controlled by SigM, V, W, X, Y), membrane proteins

This gene is a member of the following regulons

SigM regulon

The gene

Basic information

• Locus tag: BSU35840

Phenotypes of a mutant

• a tagT mutant has no phenoype, the triple tagT tagU tagV mutant is unable to grow under normal conditions PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: LytR-Cps2a-Psr family PubMed

• Paralogous protein(s): TagU, TagV

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s): Mg(2+) PubMed

• Effectors of protein activity:

• Interactions:
  • MreB-TagT PubMed

• Localization:
  • cytoplasmic membrane (with extracytoplasmic catalaytic domain) PubMed
  • extracellular (signal peptide) PubMed

Database entries

• Structure:
  • 3MEJ
  • 4DE9 PubMed

• UniProt: Q7WY78

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: ywtF PubMed

• Expression browser: tagT PubMed

• Sigma factor: SigM Pubmed

• Regulation:
  • expressed at high salt concentrations (SigM) Pubmed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: