MifM

• Description: ribosome-nascent chain sensor of membrane protein biogenesis
  
  

Gene name	mifM
Synonyms	yqzJ
Essential	no
Product	sensor of SpoIIIJ activity
Function	control of yidC2 translation
Gene expression levels in SubtiExpress: mifM
Interactions involving this protein in SubtInteract: MifM
MW, pI	11 kDa, 5.829
Gene length, protein length	285 bp, 95 aa
Immediate neighbours	polY1, yidC2
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation, membrane proteins

The gene

Basic information

• Locus tag: BSU23880

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: acts as a kind of "leader peptide" that does or does not allow translation through a hairpin structure in the yidC2 mRNA PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:
  • N-terminal transmembrane domain PubMed
  • the C-terminal domain interacts with the ribosome and arrests the own translation elongation PubMed

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • the C-terminus of MifM interacts with the ribosomal polypeptide exit tunnel PubMed
  • SpoIIIJ-MifM PubMed

• Localization:
  • membrane PubMed

Database entries

• Structure:

• UniProt: Q7WY64

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: mifM-yidC2 PubMed

• Expression browser: mifM PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • An antisense RNA is predicted for mifM PubMed
  • MifM has intrinsic sequences to arrest ribosome function, the arrest is influenced by the cotranslational engagement in the protein localization reaction PubMed
  • elongation proceeds when SpoIIIJ integrates the nascent chain of MifM into the membrane PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: