FabI

Gene name	fabI
Synonyms	yjbW
Essential	no
Product	enoyl-acyl carrier protein reductase
Function	fatty acid biosynthesis
Gene expression levels in SubtiExpress: fabI
Metabolic function and regulation of this protein in SubtiPathways: Lipid synthesis
MW, pI	27 kDa, 5.605
Gene length, protein length	774 bp, 258 aa
Immediate neighbours	thiD, cotO
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis of lipids

This gene is a member of the following regulons

FapR regulon

The gene

Basic information

• Locus tag: BSU11720

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Acyl-[acyl-carrier-protein] + NAD⁺ = trans-2,3-dehydroacyl-[acyl-carrier-protein] + NADH (according to Swiss-Prot)

• Protein family: FabI subfamily (according to Swiss-Prot)

• Paralogous protein(s): FabL, one of the two proteins has to be present for viability PubMed

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:
  • inhibited by triclosan PubMed

• Interactions:

• Localization:

Database entries

• Structure: 3OIF (complex with NAD and inhibitor triclosan) PubMed

• UniProt: P54616

• KEGG entry: [3]

• E.C. number: 1.3.1.9

Expression and regulation

• Operon: fabI PubMed

• Expression browser: fabI PubMed

• Sigma factor:

• Regulation:
  • expressed when the cells experience a lack of malonyl-CoA (FapR) PubMed
  • induced upon fatty acid biosynthesis inhibition PubMed

• Regulatory mechanism:
  • FapR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Yasutaro Fujita, Hiroshi Matsuoka, Kazutake Hirooka
Regulation of fatty acid metabolism in bacteria.
Mol Microbiol: 2007, 66(4);829-39
[PubMed:17919287] [WorldCat.org] [DOI] (P p)

Stephen W White, Jie Zheng, Yong-Mei Zhang, Rock
The structural biology of type II fatty acid biosynthesis.
Annu Rev Biochem: 2005, 74;791-831
[PubMed:15952903] [WorldCat.org] [DOI] (P p)