ThiI

• Description: sulfuryl transferase, biosynthesis of 4-thiouridine in tRNA
  
  

Gene name	thiI
Synonyms	ytbJ
Essential	no
Product	sulfuryl transferase
Function	biosynthesis of 4-thiouridine in tRNA
Interactions involving this protein in SubtInteract: ThiI
MW, pI	40 kDa, 9.663
Gene length, protein length	1074 bp, 358 aa
Immediate neighbours	sspA, nifZ
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation

The gene

Basic information

• Locus tag: BSU29580

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: receives a sulfuryl group from NifZ and transfers it to uridine at position 8 of tRNA PubMed

• Protein family: thiI family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • NifZ-ThiI PubMed

• Localization:
  • cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: O34595

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: ytbJ PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Lauren J Rajakovich, John Tomlinson, Patricia C Dos Santos
Functional Analysis of Bacillus subtilis Genes Involved in the Biosynthesis of 4-Thiouridine in tRNA.
J Bacteriol: 2012, 194(18);4933-40
[PubMed:22773787] [WorldCat.org] [DOI] (I p)

Joo-Heon Park, Pieter C Dorrestein, Huili Zhai, Cynthia Kinsland, Fred W McLafferty, Tadhg P Begley
Biosynthesis of the thiazole moiety of thiamin pyrophosphate (vitamin B1).
Biochemistry: 2003, 42(42);12430-8
[PubMed:14567704] [WorldCat.org] [DOI] (P p)