ClsA

• Description: cardiolipin synthase, major enzyme
  
  

Gene name	clsA
Synonyms	ywnE
Essential	no
Product	cardiolipin synthase, major enzyme
Function	biosynthesis of phospholipids
Metabolic function and regulation of this protein in SubtiPathways: Lipid synthesis
MW, pI	55 kDa, 9.554
Gene length, protein length	1446 bp, 482 aa
Immediate neighbours	ywnF, mta
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

biosynthesis of lipids, membrane proteins

The gene

Basic information

• Locus tag: BSU36590

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 2 Phosphatidylglycerol = diphosphatidylglycerol + glycerol (according to Swiss-Prot)

• Protein family: Cardiolipin synthase subfamily (according to Swiss-Prot)

• Paralogous protein(s): YwiE, YwjE

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cell membrane at the septum PubMed

Database entries

• Structure:

• UniProt: P71040

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: clsA PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Letal I Salzberg, John D Helmann
Phenotypic and transcriptomic characterization of Bacillus subtilis mutants with grossly altered membrane composition.
J Bacteriol: 2008, 190(23);7797-807
[PubMed:18820022] [WorldCat.org] [DOI] (I p)

Claudia S López, Alejandro F Alice, Horacio Heras, Emilio A Rivas, Carmen Sánchez-Rivas
Role of anionic phospholipids in the adaptation of Bacillus subtilis to high salinity.
Microbiology (Reading): 2006, 152(Pt 3);605-616
[PubMed:16514141] [WorldCat.org] [DOI] (P p)

Ayako Nishibori, Jin Kusaka, Hiroshi Hara, Masato Umeda, Kouji Matsumoto
Phosphatidylethanolamine domains and localization of phospholipid synthases in Bacillus subtilis membranes.
J Bacteriol: 2005, 187(6);2163-74
[PubMed:15743965] [WorldCat.org] [DOI] (P p)

Fumitaka Kawai, Momoko Shoda, Rie Harashima, Yoshito Sadaie, Hiroshi Hara, Kouji Matsumoto
Cardiolipin domains in Bacillus subtilis marburg membranes.
J Bacteriol: 2004, 186(5);1475-83
[PubMed:14973018] [WorldCat.org] [DOI] (P p)