MifM
- Description: ribosome-nascent chain sensor of membrane protein biogenesis
| Gene name | mifM |
| Synonyms | yqzJ |
| Essential | no |
| Product | sensor of SpoIIIJ activity |
| Function | control of yqjG translation |
| MW, pI | 11 kDa, 5.829 |
| Gene length, protein length | 285 bp, 95 aa |
| Immediate neighbours | polY1, yqjG |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
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Contents
Categories containing this gene/protein
translation, membrane proteins
This gene is a member of the following regulons
The MifM regulon: yqjG
The gene
Basic information
- Locus tag: BSU23880
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: acts as a kind of "leader peptide" that does or does not allow translation through a hairpin structure in the yqjG mRNA PubMed
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- the C-terminus of MifM interacts with the ribosomal polypeptide exit tunnel PubMed
- Localization: membrane PubMed
Database entries
- Structure:
- UniProt: Q7WY64
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Regulation:
- Regulatory mechanism:
- Additional information:
- An antisense RNA is predicted for mifM PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Shinobu Chiba, Takashi Kanamori, Takuya Ueda, Yoshinori Akiyama, Kit Pogliano, Koreaki Ito
Recruitment of a species-specific translational arrest module to monitor different cellular processes.
Proc Natl Acad Sci U S A: 2011, 108(15);6073-8
[PubMed:21383133]
[WorldCat.org]
[DOI]
(I p)
Irnov Irnov, Cynthia M Sharma, Jörg Vogel, Wade C Winkler
Identification of regulatory RNAs in Bacillus subtilis.
Nucleic Acids Res: 2010, 38(19);6637-51
[PubMed:20525796]
[WorldCat.org]
[DOI]
(I p)
Shinobu Chiba, Anne Lamsa, Kit Pogliano
A ribosome-nascent chain sensor of membrane protein biogenesis in Bacillus subtilis.
EMBO J: 2009, 28(22);3461-75
[PubMed:19779460]
[WorldCat.org]
[DOI]
(I p)
