YngG

• Description: 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) lyase
  
  

Gene name	yngG
Synonyms
Essential	no
Product	3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) lyase
Function	mother cell metabolism, leucine utilization
MW, pI	32 kDa, 5.753
Gene length, protein length	897 bp, 299 aa
Immediate neighbours	yngF, yngH
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU18230

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

utilization of amino acids, sporulation proteins

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: (S)-3-hydroxy-3-methylglutaryl-CoA = acetyl-CoA + acetoacetate PubMed

• Protein family: HMG-CoA lyase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1YDO

• UniProt: O34873

• KEGG entry: [3]

• E.C. number: 4.1.3.4

Expression and regulation

• Operon: yngJ-yngI-yngH-yngG-yngF-yngE PubMed

• Sigma factor: SigE PubMed

• Regulation:
  • expressed during sporulation in the mother cell (SigE) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Tzu-Lin Hsiao, Olga Revelles, Lifeng Chen, Uwe Sauer, Dennis Vitkup
Automatic policing of biochemical annotations using genomic correlations.
Nat Chem Biol: 2010, 6(1);34-40
[PubMed:19935659] [WorldCat.org] [DOI] (I p)

Raghavendra Joshi, Brian B McSpadden Gardener
Identification and Characterization of Novel Genetic Markers Associated with Biological Control Activities in Bacillus subtilis.
Phytopathology: 2006, 96(2);145-54
[PubMed:18943917] [WorldCat.org] [DOI] (P p)

Farhad Forouhar, Munif Hussain, Ramy Farid, Jordi Benach, Mariam Abashidze, William C Edstrom, Sergey M Vorobiev, Rong Xiao, Thomas B Acton, Zhuji Fu, Jung-Ja P Kim, Henry M Miziorko, Gaetano T Montelione, John F Hunt
Crystal structures of two bacterial 3-hydroxy-3-methylglutaryl-CoA lyases suggest a common catalytic mechanism among a family of TIM barrel metalloenzymes cleaving carbon-carbon bonds.
J Biol Chem: 2006, 281(11);7533-45
[PubMed:16330546] [WorldCat.org] [DOI] (P p)

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

Patrick Eichenberger, Shane T Jensen, Erin M Conlon, Christiaan van Ooij, Jessica Silvaggi, José Eduardo González-Pastor, Masaya Fujita, Sigal Ben-Yehuda, Patrick Stragier, Jun S Liu, Richard Losick
The sigmaE regulon and the identification of additional sporulation genes in Bacillus subtilis.
J Mol Biol: 2003, 327(5);945-72
[PubMed:12662922] [WorldCat.org] [DOI] (P p)