NdoA

• Description: RNase EndoA, MazF family toxin, cleaves cellular mRNAs at specific, but frequently occuring sites
  
  

Gene name	ndoA
Synonyms	ydcE
Essential	no
Product	endoribonuclease EndoA, MazF family toxin
Function	plasmid partition
MW, pI	12 kDa, 4.892
Gene length, protein length	348 bp, 116 aa
Immediate neighbours	ndoAI, rsbR
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU04660

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

Rnases, toxins, antitoxins and immunity against toxins

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: pemK family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity: the activity is inhibited by the interaction with NdoAI PubMed

• Interactions: NdoAI-NdoA PubMed

• Localization:

Database entries

• Structure: 1NE8

• UniProt: P96622

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: ndoAI-ndoA PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Shivangi Agarwal, Shivani Agarwal, Rakesh Bhatnagar
Identification and characterization of a novel toxin-antitoxin module from Bacillus anthracis.
FEBS Lett: 2007, 581(9);1727-34
[PubMed:17416361] [WorldCat.org] [DOI] (P p)

Olivier Pellegrini, Nathalie Mathy, Arhonda Gogos, Lawrence Shapiro, Ciarán Condon
The Bacillus subtilis ydcDE operon encodes an endoribonuclease of the MazF/PemK family and its inhibitor.
Mol Microbiol: 2005, 56(5);1139-48
[PubMed:15882409] [WorldCat.org] [DOI] (P p)