rbgA

rbgA
168

assembly of the 50S subunit of the ribosome, facilitates binding of EngA to the 45S assembly intermediate

Locus
BSU_16050
Molecular weight
31.83 kDa
Isoelectric point
9.53
Protein length
Gene length
Product
GTPase
Essential
yes
Synonyms
rbgA, ylqF

Genomic Context

List of homologs in different organisms, belongs to COG1161 (Galperin et al., 2021)

Gene
Coordinates
1,676,532  1,677,380
Phenotypes of a mutant
essential PubMed
depletion confers a slow growth phenotype, and induces an abnormality of the in vivo ribosome profile, with an increased level of 45S precursors and a decreased level of 70S ribosomes PubMed
premature 45S particles accumulated in RbgA-depleted cells lack several proteins, including L16, L27, L28, L33, L35 and L36 PubMed
reduced GTPase activity of RbgA results in the acquisition of RplF suppressor mutations PubMed
several 23S rRNA modifications are missing upon rbgA depletion PubMed
The protein
Catalyzed reaction/ biological activity
proper positioning of L6 in the large subunit of the ribosome prior to incorporation of L16 PubMed
unlocks the conformation adopted by 23S rRNA helices H97 and H42, which is imposed by the entry of UL6 PubMed
binds and hydrolyzes GTP and readily exchanges GDP for GTP
stabilizes rRNA helices in the A and P functional sites PubMed
Protein family
TRAFAC class YlqF/YawG GTPase family (together with CpgA and YqeH, according to UniProt)
RNA-binding domain at the C-terminus PubMed
CP-type G domain (aa 14-178) (according to UniProt)
Structure
6PVK (PDB) (complex of RbgA bound to ribosomal 50S subunit assembly intermediate) PubMed
Effectors of protein activity
GTPase activity requires binding of L16 to the 50S subunit of the ribosome PubMed
binds ppGpp PubMed, ppGpp inhibits activity PubMed
Paralogous protein(s)
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
Description
Open in new tab

rbgAylqH

2025-01-22 21:21:43

Jstuelk

103

eeb1b6a4de31a5049ef87638911db24d52f44a7c

3D86AE4ED34D50165869C1951375B5B0E636471C

Biological materials
Mutant
MGNA-B141 (ylqF::erm), available at the NBRP B. subtilis, Japan
Labs working on this gene/protein
Naotake Ogasawara, Nara, Japan
References
Reviews
Loading
Original Publications
Loading

54CD247926F7FABE4ACE85EB7021B62500B47260

Page visits: 5114

Time of last update: 2025-01-30 17:37:31

Author of last update: Jstuelk