rbgA
168
assembly of the 50S subunit of the ribosome, facilitates binding of EngA to the 45S assembly intermediate
Locus
BSU_16050
Molecular weight
31.83 kDa
Isoelectric point
9.53
Function
Product
GTPase
Essential
yes
Synonyms
rbgA, ylqF
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1161 (Galperin et al., 2021)
Gene
Coordinates
1,676,532 1,677,380
Phenotypes of a mutant
essential PubMed
depletion confers a slow growth phenotype, and induces an abnormality of the in vivo ribosome profile, with an increased level of 45S precursors and a decreased level of 70S ribosomes PubMed
The protein
Catalyzed reaction/ biological activity
unlocks the conformation adopted by 23S rRNA helices H97 and H42, which is imposed by the entry of UL6 PubMed
binds and hydrolyzes GTP and readily exchanges GDP for GTP
stabilizes rRNA helices in the A and P functional sites PubMed
Protein family
RNA-binding domain at the C-terminus PubMed
CP-type G domain (aa 14-178) (according to UniProt)
Structure
6PVK (PDB) (complex of RbgA bound to ribosomal 50S subunit assembly intermediate) PubMed
Effectors of protein activity
Paralogous protein(s)
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Biological materials
Mutant
MGNA-B141 (ylqF::erm), available at the NBRP B. subtilis, Japan
Labs working on this gene/protein
Naotake Ogasawara, Nara, Japan
References
Reviews
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Original Publications
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Page visits: 5114
Time of last update: 2025-01-30 17:37:31
Author of last update: Jstuelk