SpeA

• Description: arginine decarboxylase, required for biofilm formation
  
  

Gene name	speA
Synonyms	cad
Essential	no
Product	arginine decarboxylase
Function	spermidine, polyamine biosynthesis
Metabolic function and regulation of this protein in SubtiPathways: Cys, Met & Sulfate assimilation
MW, pI	53 kDa, 5.081
Gene length, protein length	1470 bp, 490 aa
Immediate neighbours	sr1, yktA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU14630

Phenotypes of a mutant

• no biofilm formation PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: L-arginine = agmatine + CO₂ (according to Swiss-Prot)

• Protein family: Orn/Lys/Arg decarboxylase class-I family (according to Swiss-Prot)

• Paralogous protein(s): YaaO

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P21885

• KEGG entry: [2]

• E.C. number: 4.1.1.19

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Matthew Burrell, Colin C Hanfrey, Ewan J Murray, Nicola R Stanley-Wall, Anthony J Michael
Evolution and multiplicity of arginine decarboxylases in polyamine biosynthesis and essential role in Bacillus subtilis biofilm formation.
J Biol Chem: 2010, 285(50);39224-38
[PubMed:20876533] [WorldCat.org] [DOI] (I p)

Xiao Yan Liu, Jian Lei, Xiang Liu, Xiao Dong Su, Lanfen Li
Preliminary X-ray crystallographic studies of Bacillus subtilis SpeA protein.
Acta Crystallogr Sect F Struct Biol Cryst Commun: 2009, 65(Pt 3);282-4
[PubMed:19255484] [WorldCat.org] [DOI] (I p)

A Sekowska, P Bertin, A Danchin
Characterization of polyamine synthesis pathway in Bacillus subtilis 168.
Mol Microbiol: 1998, 29(3);851-8
[PubMed:9723923] [WorldCat.org] [DOI] (P p)