YesW

• Description: rhamnogalacturonan lyase, generates oligosaccharides
  
  

Gene name	yesW
Synonyms
Essential	no
Product	rhamnogalacturonan lyase, generates oligosaccharides
Function	utilization of rhamnogalacturonan
MW, pI	67 kDa, 5.966
Gene length, protein length	1860 bp, 620 aa
Immediate neighbours	yesV, yesX
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU07050

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: FG-GAP repeat (according to Swiss-Prot)

• Paralogous protein(s): YesX

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: secreted (according to Swiss-Prot)

Database entries

• Structure: 2Z8R

• UniProt: O31526

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: yesS-yesT-yesU-yesV-yesW-yesX-yesY-yesZ PubMed

• Sigma factor:

• Regulation: induced by pectin (YesS) PubMed

• Regulatory mechanism: YesS: transcriptional activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Sandrine Poncet, Maryline Soret, Peggy Mervelet, Josef Deutscher, Philippe Noirot
Transcriptional activator YesS is stimulated by histidine-phosphorylated HPr of the Bacillus subtilis phosphotransferase system.
J Biol Chem: 2009, 284(41);28188-28197
[PubMed:19651770] [WorldCat.org] [DOI] (I p)

Akihito Ochiai, Takafumi Itoh, Bunzo Mikami, Wataru Hashimoto, Kousaku Murata
Structural determinants responsible for substrate recognition and mode of action in family 11 polysaccharide lyases.
J Biol Chem: 2009, 284(15);10181-9
[PubMed:19193638] [WorldCat.org] [DOI] (P p)

Akihito Ochiai, Takafumi Itoh, Akiko Kawamata, Wataru Hashimoto, Kousaku Murata
Plant cell wall degradation by saprophytic Bacillus subtilis strains: gene clusters responsible for rhamnogalacturonan depolymerization.
Appl Environ Microbiol: 2007, 73(12);3803-13
[PubMed:17449691] [WorldCat.org] [DOI] (P p)