YdiO

• Description: similar to DNA-3-methyladenine glycosidase II
  
  

Gene name	ydiO
Synonyms
Essential	yes PubMed
Product	unknown
Function	BsuM DNA modification
MW, pI	48 kDa, 7.659
Gene length, protein length	1281 bp, 427 aa
Immediate neighbours	ydiN, ydiP
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU06060

Phenotypes of a mutant

essential PubMed, mutation is only possible in a ydiR, ydiS, or ydjA background (i. e., in the absence of the BsuM DNA restriction system) PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: O34939

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: ydiO-ydiP PubMed

• Sigma factor:

• Regulation:
  • induced by DNA damage (LexA) PubMed

• Regulatory mechanism:
  • LexA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Nora Au, Elke Kuester-Schoeck, Veena Mandava, Laura E Bothwell, Susan P Canny, Karen Chachu, Sierra A Colavito, Shakierah N Fuller, Eli S Groban, Laura A Hensley, Theresa C O'Brien, Amish Shah, Jessica T Tierney, Louise L Tomm, Thomas M O'Gara, Alexi I Goranov, Alan D Grossman, Charles M Lovett
Genetic composition of the Bacillus subtilis SOS system.
J Bacteriol: 2005, 187(22);7655-66
[PubMed:16267290] [WorldCat.org] [DOI] (P p)

Hideyuki Ohshima, Satoshi Matsuoka, Kei Asai, Yoshito Sadaie
Molecular organization of intrinsic restriction and modification genes BsuM of Bacillus subtilis Marburg.
J Bacteriol: 2002, 184(2);381-9
[PubMed:11751814] [WorldCat.org] [DOI] (P p)