GapA

• Synonyms:

• Description: glyceraldehyde-3-phosphate dehydrogenase, (NADH-dependent), glycolytic enzyme

The gene

Basic information

• Coordinates:

• Gene length:

Phenotypes of a mutant

essential

Database entries

• DBTBS entry: [1]

• SubtiList entry:[2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: glyceraldehyde-3-phosphate dehydrogenase, (NADH-dependent)

• Protein family:

• Paralogous protein(s): GapB

Extended information on the protein

• Kinetic information

• Domains

• Modification Phosphorylation (STY)

• Effectors of protein activity:

• Interactions:
  • GapA-PtsH

HPr(Ser-46-P) binds GapA resulting in a slight inhibition of enzymatic activity.

• • GapA-Crh

Crh(Ser-46-P) binds GapA resulting in a slight inhibition of enzymatic activity.

• Localization

membrane and cytoplasm

Database entries

• Structure

• Swiss prot entry

• KEGG entry

Expression and regulation

• Operon: cggR-gapA-pgk-tpiA-pgm-eno[3]

• Sigma factor: SigA [4]

• Regulation: CggR represses the operon in the absence of glycolytic sugars [5]

• Regulatory mechanism: repression

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• Antibody:

References

1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. [6]