pupG

pupG
168

purine nucleoside phosphorylase

Locus
BSU_23490
Molecular weight
28.98 kDa
Isoelectric point
4.86
Protein length
Gene length
Function
purine salvage and interconversion
Product
purine nucleoside phosphorylase
Essential
no
E.C.
2.4.2.1
Synonyms
pupG, punA, pnp, yqkO

Genomic Context

List of homologs in different organisms, belongs to COG0005 (Galperin et al., 2021)

This gene is a member of the following regulons

SigA regulon, CcpA regulon

Gene
Coordinates
2,446,418 → 2,447,233
The protein
Catalyzed reaction/ biological activity
purine 2'-deoxy-D-ribonucleoside + phosphate --> 2-deoxy-α-D-ribose 1-phosphate + purine nucleobase (according to UniProt)
Protein family
PNP/MTAP phosphorylase family (single member, according to UniProt)
Structure
3LA8 (PDB) (from Streptococcus mutans, 57% identity)
Modification
phosphorylation on Ser-28 PubMed
secreted (according to Swiss-Prot)
Expression and Regulation
Operons
Genes
Description
Regulation
induced in the presence of nucleosides (deoxyribose 5-phosphate and ribose 5-phosphate act as molecular inducers) PubMed
Regulatory mechanism
CcpA: repression, PubMed, in ccpA regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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drmpupG

2025-02-02 10:33:49

ghost

85

14c764b577f7631beda537db900a102a9f367490

F338011D0443A78E516CD55DF097CFDB8967EE05

Biological materials
Mutant
BKE23490 (ΔpupG::erm  trpC2) available at BGSCPubMed, upstream reverse: _UP1_TCTGTCCTTCAAGAAACAGT,  downstream forward: _UP4_TAAATATGAATCAATGCAGG
BKK23490 (ΔpupG::kan  trpC2) available at BGSCPubMed, upstream reverse: _UP1_TCTGTCCTTCAAGAAACAGT,  downstream forward: _UP4_TAAATATGAATCAATGCAGG
References
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Time of last update: 2025-02-03 09:05:05

Author of last update: Melvin.boenninger