TnrA

• Description: transcriptional pleiotropic regulator invoved in global nitrogen regulation
  
  

Gene name	tnrA
Synonyms	scgR
Essential	no
Product	transcription activator/ repressor
Function	regulation of nitrogen assimilation (positive regulation of nrgAB, nasBCDEF, gabP, ureABC, guaD; negative regulation of glnRA, gltAB)
MW, pI	12 kDa, 10.235
Gene length, protein length	330 bp, 110 aa
Immediate neighbours	mgtE, ykzB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Coordinates:

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Genes/ operons controlled by TnrA

• Activation by TnrA: nrgA-nrgB

• Repression by TnrA: degS-degU, gltA-gltB

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity: feedback-inhibited GlnA prevents TnrA from DNA binding

• Interactions: TnrA-GlnA, TnrA-NrgB PubMed, TnrA-GlnA, this interaction results in loss of TnrA DNA-binding activity

• Localization:

Database entries

• Structure:

• Swiss prot entry: Q45666

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: available in the Karl Forchhammer lab

Labs working on this gene/protein

Susan Fisher, Boston, USA homepage

References

1. Wray LV Jr, Ferson AE, Rohrer K, Fisher SH (1996) TnrA, a transcription factor required for global nitrogen regulation in Bacillus subtilis. Proc Natl Acad Sci USA 93:8841-8845. PubMed
2. Yoshida K, et al. (2003) Identification of additional TnrA-regulated genes of Bacillus subtilis associated with a TnrA box. Mol Microbiol 49: 157-65. PubMed
3. Wray LV Jr, Zalieckas JM, Fisher SH. (2001) Bacillus subtilis glutamine synthetase controls gene expression through protein-protein interaction with transcription factor TnrA. Cell 107:427-435. PubMed
4. Belitsky, B. R., and Sonenshein, A. L. (2004) Modulation of activity of Bacillus subtilis regulatory proteins GltC and TnrA by glutamate dehydrogenase. J Bacteriol 186: 3399-3407.PubMed
5. Belitsky, B. R., Wray, L. V., Jr., Fisher, S. H., Bohannon, D. E. & Sonenshein, A. L. (2000). Role of TnrA in nitrogen source-dependent repression of Bacillus subtilis glutamate synthase gene expression. J Bacteriol 182, 5939-5947. PubMed
6. Heinrich, A., Woyda, K., Brauburger, K., Meiss, G., Detsch, C., Stülke, J. & Forchhammer, K. (2006) Interaction of the membrane-bound GlnK-AmtB complex with the master regulator of nitrogen metabolism TnrA in Bacillus subtilis. J. Biol. Chem. 281: 34909-34917. PubMed
7. Tojo S, Satomura T, Morisaki K, Yoshida KI, Hirooka K, Fujita Y: Negative transcriptional regulation of the ilv-leu operon for biosynthesis of branched-chain amino acids through the Bacillus subtilis global regulator TnrA. J Bacteriol 2004, 186:7971-7979. PubMed
8. Fisher, S. H., Brandenburg, J. L. & Wray, L. V. (2002). Mutations in Bacillus subtilis glutamine synthetase that block its interaction with transcription factor TnrA. Mol Microbiol 45, 627-635. PubMed
9. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed