PETM-11

pWH844: click to enlarge

• vector for the expression of proteins in E. coli, the plasmid allows to fuse a His(6) tag to the N-terminus of the expressed protein that can be cleaved of by the TEV protease

• host: E. coli BL21(DE3)/pLysS, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture

• in E. coli: ampicillin resistance

• from EMBL

• sequencing primer:
• CZ103</font>: 5’-GAGGATCGAGATCTCGATC-3‘
• CZ104: 5’-CTAGTTATTGCTCAGCGGTG-3‘