PETM-11
Revision as of 09:53, 19 November 2015 by Aklewing (talk | contribs) (Created page with "250px|thumb|right|'''pWH844: click to enlarge''' * vector for the expression of proteins in ''E. coli'', the plasmid allows to fuse a His(6) tag to t...")
- vector for the expression of proteins in E. coli, the plasmid allows to fuse a His(6) tag to the N-terminus of the expressed protein that can be cleaved of by the TEV protease
- host: E. coli BL21(DE3)/pLysS, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture
- in E. coli: ampicillin resistance
- sequencing primer:
- CZ103</font>: 5’-GAGGATCGAGATCTCGATC-3‘
- CZ104: 5’-CTAGTTATTGCTCAGCGGTG-3‘
