PrkD

• Description: protein kinase D
  
  

Gene name	prkD
Synonyms	ybdM
Essential	no
Product	protein kinase D
Function	unknown
Gene expression levels in SubtiExpress: prkD
MW, pI	29 kDa, 6.359
Gene length, protein length	768 bp, 256 aa
Immediate neighbours	ybzI, ybdN
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

protein modification, phosphoproteins

The gene

Basic information

• Locus tag: BSU02030

Database entries

• BsubCyc: BSU02030

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • ATP + a protein = ADP + a phosphoprotein (according to Swiss-Prot)
  • phosphorylates DegS on Ser-76 (in vitro) PubMed

• Protein family: protein kinase domain (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: autophosphorylated on Thr-174 (in vitro) PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU02030

• Structure:

• UniProt: O31435

• KEGG entry: [2]

• E.C. number: 2.7.11.1

Expression and regulation

• Operon:

• Expression browser: prkD PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant: GP578 (aphA3), available in the Stülke lab

• Expression vector:
  • pGP390 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), available in Stülke lab
  • for expression, purification in E. coli with N-terminal Strep-tag, in pGP172: pGP821, available in Stülke lab
  • for expression, purification in E. coli with N-terminal His-tag, in pWH844: pGP1407, available in Stülke lab

• lacZ fusion: pGP830 (in pAC7), available in Stülke lab

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Carsten Jers, Ahasanul Kobir, Elsebeth Oline Søndergaard, Peter Ruhdal Jensen, Ivan Mijakovic
Bacillus subtilis two-component system sensory kinase DegS is regulated by serine phosphorylation in its input domain.
PLoS One: 2011, 6(2);e14653
[PubMed:21304896] [WorldCat.org] [DOI] (I e)

Nico Pietack, Dörte Becher, Sebastian R Schmidl, Milton H Saier, Michael Hecker, Fabian M Commichau, Jörg Stülke
In vitro phosphorylation of key metabolic enzymes from Bacillus subtilis: PrkC phosphorylates enzymes from different branches of basic metabolism.
J Mol Microbiol Biotechnol: 2010, 18(3);129-40
[PubMed:20389117] [WorldCat.org] [DOI] (I p)

Edwige Madec, Agnieszka Laszkiewicz, Adam Iwanicki, Michal Obuchowski, Simone Séror
Characterization of a membrane-linked Ser/Thr protein kinase in Bacillus subtilis, implicated in developmental processes.
Mol Microbiol: 2002, 46(2);571-86
[PubMed:12406230] [WorldCat.org] [DOI] (P p)