SpoIVFB

• Description: intramembrane metalloprotease, processing of pro-sigma-K to active SigK
  
  

Gene name	spoIVFB
Synonyms
Essential	no
Product	intramembrane metalloprotease
Function	processing of pro-sigma-K to active SigK
Gene expression levels in SubtiExpress: spoIVFB
Interactions involving this protein in SubtInteract: SpoIVFB
MW, pI	33 kDa, 8.483
Gene length, protein length	864 bp, 288 aa
Immediate neighbours	rplU, spoIVFA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

sigma factors and their control, proteolysis, sporulation proteins, membrane proteins

This gene is a member of the following regulons

SigE regulon

The gene

Basic information

• Locus tag: BSU27970

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: processing of pro-sigma-K to active SigK PubMed

• Protein family: peptidase M50B family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:
  • C-terminal cystathionine-beta-synthase (CBS) domain, this domain binds ATP PubMed

• Modification:

• Cofactor(s):

• Effectors of protein activity: ATP regulates substrate access to the active site and renders cleavage sensitive to the cellular energy level PubMed

• Interactions:
  • SpoIVFB-proSigK PubMed
  • SpoIVFB-BofA, to inhibit proteolytic activity of SpoIVFB PubMed
  • SpoIVFB-SpoIVFA, to facilitate the recruitment of BofA to SpoIVFB PubMed

• Localization:
  • integral membrane protein PubMed
  • mother cell membrane PubMed

Database entries

• Structure:

• UniProt: P26937

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: spoIVFA-spoIVFB PubMed

• Expression browser: spoIVFB PubMed

• Sigma factor: SigE PubMed

• Regulation:
  • expressed early during sporulation in the mother cell (SigE, SpoIIID) PubMed

• Regulatory mechanism:
  • SpoIIID: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Lee Kroos, Yoshinori Akiyama
Biochemical and structural insights into intramembrane metalloprotease mechanisms.
Biochim Biophys Acta: 2013, 1828(12);2873-85
[PubMed:24099006] [WorldCat.org] [DOI] (P p)

Noël Molière, Kürşad Turgay
General and regulatory proteolysis in Bacillus subtilis.
Subcell Biochem: 2013, 66;73-103
[PubMed:23479438] [WorldCat.org] [DOI] (P p)

Gu Chen, Xu Zhang
New insights into S2P signaling cascades: regulation, variation, and conservation.
Protein Sci: 2010, 19(11);2015-30
[PubMed:20836086] [WorldCat.org] [DOI] (I p)

Michael S Wolfe
Intramembrane-cleaving proteases.
J Biol Chem: 2009, 284(21);13969-73
[PubMed:19189971] [WorldCat.org] [DOI] (P p)