YabA

• Description: inhibitor of DnaA oligomerization, tethers DnaA to the polymerase-clamp protein DnaN
  
  

Gene name	yabA
Synonyms
Essential	no
Product	negative regulator of replication initiation
Function	positioning of DnaA
Gene expression levels in SubtiExpress: yabA
Interactions involving this protein in SubtInteract: YabA
MW, pI	13 kDa, 6.065
Gene length, protein length	357 bp, 119 aa
Immediate neighbours	yaaT, yabB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA replication

The gene

Basic information

• Locus tag: BSU00330

Phenotypes of a mutant

strongly reduced growth rate in minimal medium PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • negative control of replication initiation PubMed
  • associates with the oriC region in a DnaA-dependent manner, this limits the amount of DnaA that can bind the oriC region PubMed
  • YabA inhibits co-operative DNA binding by DnaA PubMed
  • inhibits oligomerization and helix formation of DnaA PubMed

• Protein family: yabA family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains: leucine zipper

• Modification:

• Cofactor(s): Zn cluster

• Effectors of protein activity:
  • DnaN overexpression or release from the replisome decreases association of YabA with oriC, increases association of DnaA with oriC PubMed

• Interactions: DnaA-YabA-DnaN PubMed

• Localization:

Database entries

• Structure:

• UniProt: P37542

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: yabA PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Philippe Noirot, Jouy-en-Josas, France Homepage
Alan Grossman, Cambridge, MA, USA Homepage

References

Original publications

Graham Scholefield, Heath Murray
YabA and DnaD inhibit helix assembly of the DNA replication initiation protein DnaA.
Mol Microbiol: 2013, 90(1);147-59
[PubMed:23909787] [WorldCat.org] [DOI] (I p)

Houra Merrikh, Alan D Grossman
Control of the replication initiator DnaA by an anti-cooperativity factor.
Mol Microbiol: 2011, 82(2);434-46
[PubMed:21895792] [WorldCat.org] [DOI] (I p)

Alexi I Goranov, Adam M Breier, Houra Merrikh, Alan D Grossman
YabA of Bacillus subtilis controls DnaA-mediated replication initiation but not the transcriptional response to replication stress.
Mol Microbiol: 2009, 74(2);454-66
[PubMed:19737352] [WorldCat.org] [DOI] (I p)

Avigdor Eldar, Vasant K Chary, Panagiotis Xenopoulos, Michelle E Fontes, Oliver C Losón, Jonathan Dworkin, Patrick J Piggot, Michael B Elowitz
Partial penetrance facilitates developmental evolution in bacteria.
Nature: 2009, 460(7254);510-4
[PubMed:19578359] [WorldCat.org] [DOI] (I p)

Clarisse Defeu Soufo, Hervé Joël Defeu Soufo, Marie-Françoise Noirot-Gros, Astrid Steindorf, Philippe Noirot, Peter L Graumann
Cell-cycle-dependent spatial sequestration of the DnaA replication initiator protein in Bacillus subtilis.
Dev Cell: 2008, 15(6);935-41
[PubMed:19081080] [WorldCat.org] [DOI] (I p)

Marie-Françoise Noirot-Gros, M Velten, M Yoshimura, S McGovern, T Morimoto, S D Ehrlich, N Ogasawara, P Polard, Philippe Noirot
Functional dissection of YabA, a negative regulator of DNA replication initiation in Bacillus subtilis.
Proc Natl Acad Sci U S A: 2006, 103(7);2368-73
[PubMed:16461910] [WorldCat.org] [DOI] (P p)

Marie-Françoise Noirot-Gros, Etienne Dervyn, Ling Juan Wu, Peggy Mervelet, Jeffery Errington, S Dusko Ehrlich, Philippe Noirot
An expanded view of bacterial DNA replication.
Proc Natl Acad Sci U S A: 2002, 99(12);8342-7
[PubMed:12060778] [WorldCat.org] [DOI] (P p)