Gpr

• Description: spore protease (degradation of SASPs)
  
  

Gene name	gpr
Synonyms
Essential	no
Product	spore protease (degradation of SASPs)
Function	germination
Gene expression levels in SubtiExpress: gpr
MW, pI	40 kDa, 5.199
Gene length, protein length	1104 bp, 368 aa
Immediate neighbours	spoIIP, rpsT
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

germination

This gene is a member of the following regulons

SigF regulon, SigG regulon

The gene

Basic information

• Locus tag: BSU25540

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: degradation of small, acid-soluble spore proteins (SASPs)

• Protein family: peptidase A25 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P22322

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: gpr-spoIIP PubMed

• Expression browser: gpr PubMed

• Sigma factor: SigF PubMed, SigG PubMed

• Regulation: expressed during sporulation in the forespore (SigF, SigG) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Stephanie T Wang, Barbara Setlow, Erin M Conlon, Jessica L Lyon, Daisuke Imamura, Tsutomu Sato, Peter Setlow, Richard Losick, Patrick Eichenberger
The forespore line of gene expression in Bacillus subtilis.
J Mol Biol: 2006, 358(1);16-37
[PubMed:16497325] [WorldCat.org] [DOI] (P p)

Thomas M Carroll, Peter Setlow
Site-directed mutagenesis and structural studies suggest that the germination protease, GPR, in spores of Bacillus species is an atypical aspartic acid protease.
J Bacteriol: 2005, 187(20);7119-25
[PubMed:16199582] [WorldCat.org] [DOI] (P p)

C Nessi, M J Jedrzejas, P Setlow
Structure and mechanism of action of the protease that degrades small, acid-soluble spore proteins during germination of spores of Bacillus species.
J Bacteriol: 1998, 180(19);5077-84
[PubMed:9748439] [WorldCat.org] [DOI] (P p)

L B Pedersen, C Nessi, P Setlow
Most of the propeptide is dispensable for stability and autoprocessing of the zymogen of the germination protease of spores of Bacillus species.
J Bacteriol: 1997, 179(5);1824-7
[PubMed:9045848] [WorldCat.org] [DOI] (P p)

B Setlow, P Setlow
Binding to DNA protects alpha/beta-type, small, acid-soluble spore proteins of Bacillus and Clostridium species against digestion by their specific protease as well as by other proteases.
J Bacteriol: 1995, 177(14);4149-51
[PubMed:7608092] [WorldCat.org] [DOI] (P p)

B Illades-Aguiar, P Setlow
Autoprocessing of the protease that degrades small, acid-soluble proteins of spores of Bacillus species is triggered by low pH, dehydration, and dipicolinic acid.
J Bacteriol: 1994, 176(22);7032-7
[PubMed:7961468] [WorldCat.org] [DOI] (P p)

B Illades-Aguiar, P Setlow
The zymogen of the protease that degrades small, acid-soluble proteins of spores of Bacillus species can rapidly autoprocess to the active enzyme in vitro.
J Bacteriol: 1994, 176(17);5571-3
[PubMed:8071242] [WorldCat.org] [DOI] (P p)

Y Carrillo-Martinez, P Setlow
Properties of Bacillus subtilis small, acid-soluble spore proteins with changes in the sequence recognized by their specific protease.
J Bacteriol: 1994, 176(17);5357-63
[PubMed:8071212] [WorldCat.org] [DOI] (P p)

B Illades-Aguiar, P Setlow
Studies of the processing of the protease which initiates degradation of small, acid-soluble proteins during germination of spores of Bacillus species.
J Bacteriol: 1994, 176(10);2788-95
[PubMed:8188581] [WorldCat.org] [DOI] (P p)

J L Sanchez-Salas, P Setlow
Proteolytic processing of the protease which initiates degradation of small, acid-soluble proteins during germination of Bacillus subtilis spores.
J Bacteriol: 1993, 175(9);2568-77
[PubMed:8478323] [WorldCat.org] [DOI] (P p)

J L Sanchez-Salas, M L Santiago-Lara, B Setlow, M D Sussman, P Setlow
Properties of Bacillus megaterium and Bacillus subtilis mutants which lack the protease that degrades small, acid-soluble proteins during spore germination.
J Bacteriol: 1992, 174(3);807-14
[PubMed:1732215] [WorldCat.org] [DOI] (P p)

M D Sussman, P Setlow
Cloning, nucleotide sequence, and regulation of the Bacillus subtilis gpr gene, which codes for the protease that initiates degradation of small, acid-soluble proteins during spore germination.
J Bacteriol: 1991, 173(1);291-300
[PubMed:1840582] [WorldCat.org] [DOI] (P p)