YsbA

• Description: putative anti-holin
  
  

Gene name	ysbA
Synonyms	lrgA
Essential	no
Product	putative anti-holin
Function	unknown
Gene expression levels in SubtiExpress: ysbA
MW, pI	15 kDa, 9.825
Gene length, protein length	438 bp, 146 aa
Immediate neighbours	ysbB, lytT
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

membrane proteins, proteins of unknown function

The gene

Basic information

• Locus tag: BSU28910

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: LrgA subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P94515

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: ysbA-ysbB PubMed

• Expression browser: ysbA PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • the mRNA is substantially stabilized upon depletion of RNase Y PubMed
  • the expression of the ysbA-ysbB operon is strongly increased in a cshA mutant PubMed

Biological materials

• Mutant:
  • GP1554 (ysbA-ysbB::spc), available in Jörg Stülke's lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Lehnik-Habrink M, Schaffer M, Mäder U, Diethmaier C, Herzberg C, Stülke J  
RNA processing in Bacillus subtilis: identification of targets of the essential RNase Y. 
Mol Microbiol. 2011 81(6): 1459-1473. 
PubMed:21815947

Kelly C Rice, Ethan E Mann, Jennifer L Endres, Elizabeth C Weiss, James E Cassat, Mark S Smeltzer, Kenneth W Bayles
The cidA murein hydrolase regulator contributes to DNA release and biofilm development in Staphylococcus aureus.
Proc Natl Acad Sci U S A: 2007, 104(19);8113-8
[PubMed:17452642] [WorldCat.org] [DOI] (P p)

Kelly C Rice, Jeremy B Nelson, Toni G Patton, Soo-Jin Yang, Kenneth W Bayles
Acetic acid induces expression of the Staphylococcus aureus cidABC and lrgAB murein hydrolase regulator operons.
J Bacteriol: 2005, 187(3);813-21
[PubMed:15659658] [WorldCat.org] [DOI] (P p)