YitJ

• Description: methionine synthase
  
  

Gene name	yitJ
Synonyms
Essential	no
Product	methionine synthase
Function	methionine biosynthesis
Gene expression levels in SubtiExpress: yitJ
Metabolic function and regulation of this protein in SubtiPathways: Cys, Met & Sulfate assimilation
MW, pI	67 kDa, 5.336
Gene length, protein length	1836 bp, 612 aa
Immediate neighbours	yitI, yitK
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

membrane proteins, biosynthesis/ acquisition of amino acids

This gene is a member of the following regulons

S-box

The gene

Basic information

• Locus tag: BSU11010

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: membrane associated PubMed

Database entries

• Structure:

• UniProt: O06745

• KEGG entry: [2]

• E.C. number: 2.1.1.13

Expression and regulation

• Operon: yitJ-yitI-yitH PubMed

• Expression browser: yitJ PubMed

• Sigma factor:

• Regulation:
  • induced by methionine starvation (S-box) PubMed
  • repressed by casamino acids PubMed

• Regulatory mechanism: S-box: transcription termination/ antitermination, the S-box riboswitch binds S-adenosylmethionine resulting in termination PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

The S-box

Vamsi Krishna Boyapati, Wei Huang, Jessica Spedale, Fareed Aboul-Ela
Basis for ligand discrimination between ON and OFF state riboswitch conformations: the case of the SAM-I riboswitch.
RNA: 2012, 18(6);1230-43
[PubMed:22543867] [WorldCat.org] [DOI] (I p)

Changrui Lu, Fang Ding, Anirban Chowdhury, Vineeta Pradhan, Jerneja Tomsic, W Michael Holmes, Tina M Henkin, Ailong Ke
SAM recognition and conformational switching mechanism in the Bacillus subtilis yitJ S box/SAM-I riboswitch.
J Mol Biol: 2010, 404(5);803-18
[PubMed:20951706] [WorldCat.org] [DOI] (I p)

Ana Gutiérrez-Preciado, Tina M Henkin, Frank J Grundy, Charles Yanofsky, Enrique Merino
Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.
Microbiol Mol Biol Rev: 2009, 73(1);36-61
[PubMed:19258532] [WorldCat.org] [DOI] (I p)

Jerneja Tomsic, Brooke A McDaniel, Frank J Grundy, Tina M Henkin
Natural variability in S-adenosylmethionine (SAM)-dependent riboswitches: S-box elements in bacillus subtilis exhibit differential sensitivity to SAM In vivo and in vitro.
J Bacteriol: 2008, 190(3);823-33
[PubMed:18039762] [WorldCat.org] [DOI] (I p)

F J Grundy, T M Henkin
The S box regulon: a new global transcription termination control system for methionine and cysteine biosynthesis genes in gram-positive bacteria.
Mol Microbiol: 1998, 30(4);737-49
[PubMed:10094622] [WorldCat.org] [DOI] (P p)