MifM

• Description: ribosome-nascent chain sensor of membrane protein biogenesis
  
  

Gene name	mifM
Synonyms	yqzJ
Essential	no
Product	sensor of SpoIIIJ activity
Function	control of yqjG translation
MW, pI	11 kDa, 5.829
Gene length, protein length	285 bp, 95 aa
Immediate neighbours	polY1, yqjG
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation, membrane proteins

The gene

Basic information

• Locus tag: BSU23880

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: acts as a kind of "leader peptide" that does or does not allow translation through a hairpin structure in the yqjG mRNA PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • the C-terminus of MifM interacts with the ribosomal polypeptide exit tunnel PubMed

• Localization: membrane PubMed

Database entries

• Structure:

• UniProt: Q7WY64

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: mifM PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • An antisense RNA is predicted for mifM PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Gene-Wei Li, Eugene Oh, Jonathan S Weissman
The anti-Shine-Dalgarno sequence drives translational pausing and codon choice in bacteria.
Nature: 2012, 484(7395);538-41
[PubMed:22456704] [WorldCat.org] [DOI] (I e)

Shinobu Chiba, Takashi Kanamori, Takuya Ueda, Yoshinori Akiyama, Kit Pogliano, Koreaki Ito
Recruitment of a species-specific translational arrest module to monitor different cellular processes.
Proc Natl Acad Sci U S A: 2011, 108(15);6073-8
[PubMed:21383133] [WorldCat.org] [DOI] (I p)

Irnov Irnov, Cynthia M Sharma, Jörg Vogel, Wade C Winkler
Identification of regulatory RNAs in Bacillus subtilis.
Nucleic Acids Res: 2010, 38(19);6637-51
[PubMed:20525796] [WorldCat.org] [DOI] (I p)

Shinobu Chiba, Anne Lamsa, Kit Pogliano
A ribosome-nascent chain sensor of membrane protein biogenesis in Bacillus subtilis.
EMBO J: 2009, 28(22);3461-75
[PubMed:19779460] [WorldCat.org] [DOI] (I p)