Tkt

• Description: transketolase
  
  

Gene name	tkt
Synonyms	tktA
Essential	no
Product	transketolase
Function	pentose phosphate pathway
Interactions involving this protein in SubtInteract: Tkt
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism
MW, pI	72 kDa, 4.803
Gene length, protein length	2001 bp, 667 aa
Immediate neighbours	ynzC, sirA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

carbon core metabolism, phosphoproteins

This gene is a member of the following regulons

Spo0A regulon

The gene

Basic information

• Locus tag: BSU17890

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-ribose 5-phosphate + D-xylulose 5-phosphate (according to Swiss-Prot)

• Protein family: transketolase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylated on ser/ thr/ tyr PubMed

• Cofactor(s): thiamine pyrophosphate

• Effectors of protein activity:

• Interactions: Tkt-Tpi PubMed

• Localization:

Database entries

• Structure: [3K95], complex with thiamine diphosphate, from B. anthracis

• UniProt: P45694

• KEGG entry: [3]

• E.C. number: 2.2.1.1

Expression and regulation

• Operon: tkt PubMed

• Sigma factor:

• Regulation:
  • repressed under conditions that trigger sporulation (Spo0A) PubMed

• Regulatory mechanism:
  • Spo0A: transcription repression PubMed

• Additional information:

Biological materials

• Mutant: BS4530 (aphA3), available in Stülke lab

• Expression vector:
  • pGP94 (N-terminal Strep-tag, for SPINE, expression in B. subtilis, in pGP380), available in Stülke lab
  • for expression, purification in E. coli with N-terminal Strep-tag, in pGP172: pGP820, available in Stülke lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab

• Antibody:

• FLAG-tag construct: GP1406 (spc, based on pGP1331), available in the Stülke lab

References

Reviews

G Schenk, R G Duggleby, P F Nixon
Properties and functions of the thiamin diphosphate dependent enzyme transketolase.
Int J Biochem Cell Biol: 1998, 30(12);1297-318
[PubMed:9924800] [WorldCat.org] [DOI] (P p)