Nth

• Description: endonuclease III, removes abasic sites during base excision repair
  
  

Gene name	nth
Synonyms	jooB, ypoB
Essential	no
Product	endonuclease III
Function	DNA repair
MW, pI	24 kDa, 9.277
Gene length, protein length	657 bp, 219 aa
Immediate neighbours	ypoC, dnaD
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

DNA repair/ recombination

The gene

Basic information

• Locus tag: BSU22340

Expression

Phenotypes of a mutant

• the mutant is sensititive to exposure to H2O2 PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate (according to Swiss-Prot)

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:
  • activity is stimulated by untwisting of the DNA (by DnaD, Hbs, or YonN) PubMed

• Interactions:

• Localization:

Database entries

• Structure: 1P59 (complex with DNA, Geobacillus stearothermophilus)

• UniProt: P39788

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: dnaD-nth PubMed

• Sigma factor:

• Regulation:
  • constitutively expressed PubMed
  • expression is transiently increased after treatment with H2O2 PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Christopher Collier, Cristina Machón, Geoff S Briggs, Wiep Klaas Smits, Panos Soultanas
Untwisting of the DNA helix stimulates the endonuclease activity of Bacillus subtilis Nth at AP sites.
Nucleic Acids Res: 2012, 40(2);739-50
[PubMed:21954439] [WorldCat.org] [DOI] (I p)

J Christopher Fromme, Gregory L Verdine
Structure of a trapped endonuclease III-DNA covalent intermediate.
EMBO J: 2003, 22(13);3461-71
[PubMed:12840008] [WorldCat.org] [DOI] (P p)