DeoD

• Description: purine nucleoside phosphorylase
  
  

Gene name	deoD
Synonyms
Essential	no
Product	purine nucleoside phosphorylase
Function	purine salvage and interconversion
Metabolic function and regulation of this protein in SubtiPathways: Purine salvage, Nucleoside catabolism
MW, pI	25 kDa, 4.978
Gene length, protein length	699 bp, 233 aa
Immediate neighbours	yodJ, yodL
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

biosynthesis/ acquisition of nucleotides, membrane proteins

The gene

Basic information

• Locus tag: BSU19630

Expression

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate (according to Swiss-Prot)

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • membrane (according to Swiss-Prot)

Database entries

• Structure: 1XE3 (from Bacillus anthracis, 71% identity, 85% similarity) PubMed

• UniProt: O34925

• KEGG entry: [2]

• E.C. number: 2.4.2.1

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Haojian Li, Guoqiang Zhang, Aihua Deng, Ning Chen, Tingyi Wen
De novo engineering and metabolic flux analysis of inosine biosynthesis in Bacillus subtilis.
Biotechnol Lett: 2011, 33(8);1575-80
[PubMed:21424839] [WorldCat.org] [DOI] (I p)

Rosa Grenha, Vladimir M Levdikov, Mark J Fogg, Elena V Blagova, James A Brannigan, Anthony J Wilkinson, Keith S Wilson
Structure of purine nucleoside phosphorylase (DeoD) from Bacillus anthracis.
Acta Crystallogr Sect F Struct Biol Cryst Commun: 2005, 61(Pt 5);459-62
[PubMed:16511068] [WorldCat.org] [DOI] (I p)