PnbA

• Description: para-nitrobenzyl esterase
  
  

Gene name	pnbA
Synonyms	estB
Essential	no
Product	para-nitrobenzyl esterase
Function	lipid degradation
MW, pI	53 kDa, 4.776
Gene length, protein length	1467 bp, 489 aa
Immediate neighbours	slrR, padC
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

utilization of lipids, phosphoproteins

This gene is a member of the following regulons

AbrB regulon

The gene

Basic information

• Locus tag: BSU34390

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Triacylglycerol + H₂O = diacylglycerol + a carboxylate (according to Swiss-Prot)

• Protein family: type-B carboxylesterase/lipase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylation on Ser-189 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1C7J PubMed

• UniProt: P37967

• KEGG entry: [2]

• E.C. number: 3.1.1.3 3.1.1.1]

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:
  • repressed during logarithmic growth (AbrB) PubMed

• Regulatory mechanism:
  • AbrB: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:
  • GP951 (pnbA::cat), available in Stülke lab
  • GP955 (slrR-pnbA::cat), available in Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: