ThrB

• Description: homoserine kinase
  
  

Gene name	thrB
Synonyms	thrA
Essential	no
Product	homoserine kinase
Function	biosynthesis of threonine
Metabolic function and regulation of this protein in SubtiPathways: Lys, Thr
MW, pI	33 kDa, 4.735
Gene length, protein length	927 bp, 309 aa
Immediate neighbours	yuxL, thrC
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU32240

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + L-homoserine = ADP + O-phospho-L-homoserine (according to Swiss-Prot)

• Protein family: Homoserine kinase subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 3HUL (from Listeria monocytogenes, 41% identity, 61% similarity)

• UniProt: P04948

• KEGG entry: [2]

• E.C. number: 2.7.1.39

Expression and regulation

• Operon: hom-thrC-thrB PubMed

• Sigma factor:

• Regulation: repressed by casamino acids PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J Bacteriol: 2002, 184(15);4288-95
[PubMed:12107147] [WorldCat.org] [DOI] (P p)

C Parsot
Evolution of biosynthetic pathways: a common ancestor for threonine synthase, threonine dehydratase and D-serine dehydratase.
EMBO J: 1986, 5(11);3013-9
[PubMed:3098560] [WorldCat.org] [DOI] (P p)