RapH

• Description: response regulator aspartate phosphatase, dephosphorylates Spo0F-P, control of the phosphorelay, control of ComA activity
  
  

Gene name	rapH
Synonyms	yeeH, yzqA
Essential	no
Product	response regulator aspartate phosphatase
Function	control of sporulation initiation and ComA activity
MW, pI	49 kDa, 6.121
Gene length, protein length	1293 bp, 431 aa
Immediate neighbours	yeeG, yeeI
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU06830

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: binds to and thereby inhibits ComA activity PubMed; in addition, RapH dephosphorylates Spo0F-P and thereby controls the phosphorelay PubMed

• Protein family: RAP family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: RapH-ComA PubMed, RapH-Spo0F-P PubMed

• Localization:

Database entries

• Structure:

• UniProt: P40771

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: rapH PubMed

• Sigma factor:

• Regulation:
  • repressed by RghR PubMed

• Regulatory mechanism:
  • RghR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Wiep Klaas Smits, Cristina Bongiorni, Jan-Willem Veening, Leendert W Hamoen, Oscar P Kuipers, Marta Perego
Temporal separation of distinct differentiation pathways by a dual specificity Rap-Phr system in Bacillus subtilis.
Mol Microbiol: 2007, 65(1);103-20
[PubMed:17581123] [WorldCat.org] [DOI] (P p)

Kentaro Hayashi, Tsukahara Kensuke, Kazuo Kobayashi, Naotake Ogasawara, Mitsuo Ogura
Bacillus subtilis RghR (YvaN) represses rapG and rapH, which encode inhibitors of expression of the srfA operon.
Mol Microbiol: 2006, 59(6);1714-29
[PubMed:16553878] [WorldCat.org] [DOI] (P p)