RacA

• Description: cell division protein: attaches the chromosome to the cell pole
  
  

Gene name	racA
Synonyms	ywkC
Essential	no
Product	cell division protein
Function	cell division
MW, pI	20 kDa, 9.514
Gene length, protein length	552 bp, 184 aa
Immediate neighbours	ywkD, ywkB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU37030

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: racA family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: DisA

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P45870

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:
  • expressed under conditions that trigger sporulation (Spo0A) PubMed

• Regulatory mechanism:
  • Spo0A: transcription activation PubMed

• Additional information:

Biological materials

• Mutant: TMB042 (erm), available in the Thorsten Mascher lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Rok Lenarcic, Sven Halbedel, Loek Visser, Michael Shaw, Ling Juan Wu, Jeff Errington, Davide Marenduzzo, Leendert W Hamoen
Localisation of DivIVA by targeting to negatively curved membranes.
EMBO J: 2009, 28(15);2272-82
[PubMed:19478798] [WorldCat.org] [DOI] (I p)

Sigal Ben-Yehuda, Masya Fujita, Xiaole Shirley Liu, Boris Gorbatyuk, Dunja Skoko, Jie Yan, John F Marko, Jun S Liu, Patrick Eichenberger, David Z Rudner, Richard Losick
Defining a centromere-like element in Bacillus subtilis by Identifying the binding sites for the chromosome-anchoring protein RacA.
Mol Cell: 2005, 17(6);773-82
[PubMed:15780934] [WorldCat.org] [DOI] (P p)

Masaya Fujita, José Eduardo González-Pastor, Richard Losick
High- and low-threshold genes in the Spo0A regulon of Bacillus subtilis.
J Bacteriol: 2005, 187(4);1357-68
[PubMed:15687200] [WorldCat.org] [DOI] (P p)

Virginie Molle, Masaya Fujita, Shane T Jensen, Patrick Eichenberger, José E González-Pastor, Jun S Liu, Richard Losick
The Spo0A regulon of Bacillus subtilis.
Mol Microbiol: 2003, 50(5);1683-701
[PubMed:14651647] [WorldCat.org] [DOI] (P p)

Ling Juan Wu, Jeff Errington
RacA and the Soj-Spo0J system combine to effect polar chromosome segregation in sporulating Bacillus subtilis.
Mol Microbiol: 2003, 49(6);1463-75
[PubMed:12950914] [WorldCat.org] [DOI] (P p)

Sigal Ben-Yehuda, David Z Rudner, Richard Losick
RacA, a bacterial protein that anchors chromosomes to the cell poles.
Science: 2003, 299(5606);532-6
[PubMed:12493822] [WorldCat.org] [DOI] (I p)