PbuG

• Description: hypoxanthin/ guanine permease
  
  

Gene name	pbuG
Synonyms	yebB
Essential	no
Product	hypoxanthin/ guanine permease
Function	hypoxanthine and guanine uptake
Metabolic function and regulation of this protein in SubtiPathways: Purine salvage, Nucleotides (regulation)
MW, pI	46 kDa, 8.808
Gene length, protein length	1320 bp, 440 aa
Immediate neighbours	guaA, yebC
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU06370

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s): PbuO

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: membrane PubMed

Database entries

• Structure: 2EEW (the pbuG riboswitch, U47c mutant, bound to hypoxanthine)

• Swiss prot entry: O34987

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: pbuG

• Sigma factor:

• Regulation:
  • repressed in the presence of purine nucleotides (PurR) PubMed
  • repressed by hypoxanthine and guanine, PurR-independent, (G-box)PubMed
  • repressed in the presence of adenine or adenosine(PurR) PubMed

• Regulatory mechanism:
  • PurR: transcription repression PubMed
  • PurR: transcription repression (molecular inducer: PRPP) PubMed
  • G-box: transcription termination/ antitermination (riboswitch) PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: