QdoI

• Description: Fe-containing quercetin 2,3-dioxygenase
  
  

Gene name	qdoI
Synonyms	yxaG
Essential	no
Product	Fe-containing quercetin 2,3-dioxygenase
Function	utilization of the abundant plant product quercetin
MW, pI	37,4 kDa, 5.562
Gene length, protein length	1011 bp, 337 aa
Immediate neighbours	yxaH, yxaF
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag:

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Quercetin + O₂ = 2-(3,4-dihydroxybenzoyloxy)-4,6-dihydroxybenzoate + CO + H⁺ (according to Swiss-Prot)

• Protein family:bicupin family

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s): Fe(2+)

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 2H0V

• Swiss prot entry: P42106

• KEGG entry: BSU39980

• E.C. number:

Expression and regulation

• Operon: qdoI-yxaH PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

1. Bowater et al. (2004) Bacillus subtilis YxaG is a novel Fe-containing quercetin 2,3-dioxygenase. FEBS Lett. 557: 45-48. PubMed