cheY

cheY
168

two-component response regulator, modulation of flagellar switch bias, CheY-phosphate induces a switch from clockwise to counter-clockwise rotation

Locus
BSU_16330
Molecular weight
13.17 kDa
Isoelectric point
4.75
Protein length
120 aaSequenceBlast
Gene length
363 bpSequenceBlast
Function
modulation of flagellar switch bias
Product
two-component response regulator
Essential
no
Synonyms
cheY, cheB
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG2204 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,703,834  1,704,196
Phenotypes of a mutant
not essential for pellicle biofilm formation, but mutant is outcompeted by the wild-type strain when competed during pellicle formation PubMed
defective in Swarming motility PubMed
The protein
Domains
Response regulatory domain (aa 4-119) (according to UniProt)
Structure
1TMY (PDB) (CheY from Thermotoga maritima)
P24072 (AlphaFold)
Modification
phosphorylated by CheA on an Asp residue
dephosphorylated by CheC PubMed
Paralogous protein(s)
YneI
Localization
cytoplasm (according to UniProt)
Expression and Regulation
Operons
Genes
ylxF-fliK-flgD-flgE-swrD-fliL-fliM-fliY-cheY-fliO-fliP-fliQ-fliR-flhB-flhA-flhF-flhG-cheB-cheA-cheW-cheC-cheD-sigD-swrB
Description
PubMed
Regulation
see fla-che operon
Regulatory mechanism
Spo0A: repression, in spo0A regulon
CodY: repression, in codY regulon
DegU: repression, in degU regulon
SwrA: activation, in swrA regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
SigD: sigma factor, PubMed, in sigD regulon
Open in new tab

ylxF→swrB

2025-04-04 21:21:07

Jstuelk

170

EECFE9022742ECC57D9BBA5CE7DB7A4D1BFD42A8

0535E3D13CBD000A94D62050515BF8D1D5E3F011

Genes
flgB-flgC-fliE-fliF-fliG-fliH-fliI-fliJ-ylxF-fliK-flgD-flgE-swrD-fliL-fliM-fliY-cheY-fliO-fliP-fliQ-fliR-flhB-flhA-flhF-flhG-cheB-cheA-cheW-cheC-cheD-sigD-swrB
Description
PubMed
Regulation
expression during spore germination is strongly reduced under conditions of osmotic stress PubMed
Regulatory mechanism
Spo0A: repression, in spo0A regulon
SwrA: activation, in swrA regulon
CodY: repression, in codY regulon
DegU: repression, in degU regulon
SinR: transcription elongation, binding of the SinR-SlrR heteromer to sites within fliE and fliI results in inhibition of transcription elongation PubMed, in sinR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
SigD: sigma factor, PubMed, in sigD regulon
Open in new tab

flgB→swrB

2025-04-03 01:56:48

Jstuelk

173

024d59ae468aa14568753f57122b789c39ed6c06

4D920B925DA071AE7390E022B9AB625E40B7B2AC

Biological materials
Mutant
1A798 ( cheY::cat), PubMed, available at BGSC
DS6870 (marker-less in NCIB3610) PubMed
BKE16330 (cheY::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAATCTATCTCTCTCCTT,  downstream forward: _UP4_TTAAATTAAAGGGTGTACGA
BKK16330 (cheY::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAATCTATCTCTCTCCTT,  downstream forward: _UP4_TTAAATTAAAGGGTGTACGA
References
Reviews
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Original Publications
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2F0495C7EAB81BDB1F3181677C555537BD2A972F

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Time of last update: 2025-04-11 22:00:07

Author of last update: Jstuelk