CheR

• Description: MCPs methyltransferase
  
  

Gene name	cheR
Synonyms
Essential	no
Product	MCPs methyltransferase
Function	motility, chemotaxis
Gene expression levels in SubtiExpress: cheR
MW, pI	29 kDa, 9.253
Gene length, protein length	768 bp, 256 aa
Immediate neighbours	aroF, ndk
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

protein modification, motility and chemotaxis

The gene

Basic information

• Locus tag: BSU22720

Database entries

• BsubCyc: BSU22720

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: S-adenosyl-L-methionine + protein L-glutamate = S-adenosyl-L-homocysteine + protein L-glutamate methyl ester (according to Swiss-Prot)

• Protein family: Glu/Leu/Phe/Val dehydrogenases family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU22720

• Structure:

• UniProt: P31105

• KEGG entry: [2]

• E.C. number: 2.1.1.80

Expression and regulation

• Operon:

• Expression browser: cheR PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • in minimal medium, CheR is present with 1,100 +/- 280 molecules per cell PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 128 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 302 PubMed

Biological materials

• Mutant:
  • 1A830 ( cheR::cat), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Original publications

Monu Batra, Rajesh Sharma, Vemika Chandra, Megha Aggarwal, Uday Agarwal, Pawan Gupta, Rajesh Pratap Singh, Shailly Tomar
In silico and proteomic analysis of protein methyltransferase CheR from Bacillus subtilis.
Int J Biol Macromol: 2015, 77;168-80
[PubMed:25799883] [WorldCat.org] [DOI] (I p)

Vincent J Cannistraro, George D Glekas, Christopher V Rao, George W Ordal
Cellular stoichiometry of the chemotaxis proteins in Bacillus subtilis.
J Bacteriol: 2011, 193(13);3220-7
[PubMed:21515776] [WorldCat.org] [DOI] (I p)

M M Rosario, G W Ordal
CheC and CheD interact to regulate methylation of Bacillus subtilis methyl-accepting chemotaxis proteins.
Mol Microbiol: 1996, 21(3);511-8
[PubMed:8866475] [WorldCat.org] [DOI] (P p)