YwlE

• Description: protein arginine phosphatase
  
  

Gene name	ywlE
Synonyms	ipc-31d
Essential	no
Product	protein arginine phosphatase
Function	dephosphorylation of McsB
Gene expression levels in SubtiExpress: ywlE
MW, pI	16 kDa, 6.681
Gene length, protein length	450 bp, 150 aa
Immediate neighbours	ywlF, ywlD
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

protein modification

The gene

Basic information

• Locus tag: BSU36930

Database entries

• BsubCyc: BSU36930

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Protein arginine phosphate + H₂O = protein arginine + phosphate PubMed

• Protein family:

• Paralogous protein(s): YfkJ

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • McsB-YwlE (dephosphorylation of McsB) PubMed

• Localization: weak oligomerization, results in inactivation of YwlE PubMed

Database entries

• BsubCyc: BSU36930

• Structure: 1ZGG

• UniProt: P39155

• KEGG entry: [2]

• E.C. number: 3.1.3.48

Expression and regulation

• Operon:

• Expression browser: ywlE PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 91 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 272 PubMed

Biological materials

• Mutant:
  • ywlE::aphA3 available from Ulf Gerth's lab
  • ywlE::tet available from Ulf Gerth's lab
  • ywlE::spec available from Ulf Gerth's lab
  • GP1458 (ywlE::aphA3), available in Jörg Stülke's lab
  • GP1459 (ywlE::tet), available in Jörg Stülke's lab

• Expression vector: for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Ulf Gerth's lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: available in Ulf Gerth's lab

Labs working on this gene/protein

• Ulf Gerth, Greifswald, Germany

• Fabian Commichau Göttingen, Germany