AspB

• Description: aspartate transaminase
  
  

Gene name	aspB
Synonyms
Essential	no
Product	aspartate transaminase
Function	biosynthesis of aspartate
Gene expression levels in SubtiExpress: aspB
Metabolic function and regulation of this protein in SubtiPathways: aspB
MW, pI	42 kDa, 5.298
Gene length, protein length	1179 bp, 393 aa
Immediate neighbours	asnS, ypmB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, most abundant proteins

The gene

Basic information

• Locus tag: BSU22370

Database entries

• BsubCyc: BSU22370

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate (according to Swiss-Prot)

• Protein family: TCR/tet family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU22370

• Structure: 1J32 (from Phormidium lapideum, 45% identity, 66% similarity)

• UniProt: P53001

• KEGG entry: [2]

• E.C. number: 2.6.1.1

Expression and regulation

• Operon: ypmB-aspB

• Expression browser: aspB PubMed

• Sigma factor:

• Regulation: constitutive

• Regulatory mechanism:

• Additional information:
  • belongs to the 100 most abundant proteins PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Christine Eymann, Annette Dreisbach, Dirk Albrecht, Jörg Bernhardt, Dörte Becher, Sandy Gentner, Le Thi Tam, Knut Büttner, Gerrit Buurman, Christian Scharf, Simone Venz, Uwe Völker, Michael Hecker
A comprehensive proteome map of growing Bacillus subtilis cells.
Proteomics: 2004, 4(10);2849-76
[PubMed:15378759] [WorldCat.org] [DOI] (P p)