WalJ

• Description: coordination of cell division with DNA replication
  
  

Gene name	walJ
Synonyms	yycJ
Essential	no
Product	unknown
Function	coordination of cell division with DNA replication
Gene expression levels in SubtiExpress: walJ
MW, pI	29 kDa, 5.503
Gene length, protein length	804 bp, 268 aa
Immediate neighbours	htrC, yycI
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell division, cell wall synthesis

The gene

Basic information

• Locus tag: BSU40370

Phenotypes of a mutant

• the mutant divides over unsegregated chromosomes more frequently than wild-type cells, and this phenotype is exacerbated when DNA replication is inhibited PubMed
• increased sensitivity to a narrow spectrum of cephalosporin antibiotics PubMed
• disruption of the corresponding gene in B. anthracis results in a mutator phenotype PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: the enzymatic activity of WalJ directly or indirectly affects cell wall metabolism and is required for accurate coordination of cell division with DNA replication PubMed

• Protein family: predicted member of the metallo-β-lactamase superfamily

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: C0SP91

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: walR-walK-yycH-yycI-walJ-htrC PubMed

• Expression browser: walJ PubMed

• Sigma factor:

• Regulation: expressed during vegetative growth, repressed during stationary phase PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • 1A1044 ( walJ::spec), PubMed, available at BGSC
  • 1A876 ( walJ::spec), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Hanjing Yang, Cameron Sikavi, Katherine Tran, Shauna M McGillivray, Victor Nizet, Madeline Yung, Aileen Chang, Jeffrey H Miller
Papillation in Bacillus anthracis colonies: a tool for finding new mutators.
Mol Microbiol: 2011, 79(5);1276-93
[PubMed:21205011] [WorldCat.org] [DOI] (I p)

Steven J Biller, Kyle J Wayne, Malcolm E Winkler, William F Burkholder
The putative hydrolase YycJ (WalJ) affects the coordination of cell division with DNA replication in Bacillus subtilis and may play a conserved role in cell wall metabolism.
J Bacteriol: 2011, 193(4);896-908
[PubMed:21169496] [WorldCat.org] [DOI] (I p)

C Fabret, J A Hoch
A two-component signal transduction system essential for growth of Bacillus subtilis: implications for anti-infective therapy.
J Bacteriol: 1998, 180(23);6375-83
[PubMed:9829949] [WorldCat.org] [DOI] (P p)