YdaK

• Description: general stress protein, may act as c-di-GMP receptor
  
  

Gene name	ydaK
Synonyms
Essential	no
Product	general stress protein
Function	unknown
Gene expression levels in SubtiExpress: ydaK
MW, pI	32 kDa, 6.695
Gene length, protein length	849 bp, 283 aa
Immediate neighbours	ydaJ, ydaL
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

general stress proteins (controlled by SigB)

This gene is a member of the following regulons

SigB regulon

The gene

Basic information

• Locus tag: BSU04280

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:
  • contains a C-terminal degenerated GGDEF domain PubMed

• Modification:

• Cofactor(s):
  • binds c-di-GPM at high concentration (K(D) for c-di-GMP: 1.1 myM) PubMed

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P96585

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: ydaJ-ydaK-ydaL-ydaM-ydaN PubMed

• Expression browser: ydaK PubMed

• Sigma factor: SigB PubMed

• Regulation:
  • expressed during stress (SigB) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant: GP1592 (kan) available in Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Xiaohui Gao, Sampriti Mukherjee, Paige M Matthews, Loubna A Hammad, Daniel B Kearns, Charles E Dann
Functional characterization of core components of the Bacillus subtilis cyclic-di-GMP signaling pathway.
J Bacteriol: 2013, 195(21);4782-92
[PubMed:23893111] [WorldCat.org] [DOI] (I p)

Yun Chen, Yunrong Chai, Jian-hua Guo, Richard Losick
Evidence for cyclic Di-GMP-mediated signaling in Bacillus subtilis.
J Bacteriol: 2012, 194(18);5080-90
[PubMed:22821967] [WorldCat.org] [DOI] (I p)

Pierre Nicolas, Ulrike Mäder, Etienne Dervyn, Tatiana Rochat, Aurélie Leduc, Nathalie Pigeonneau, Elena Bidnenko, Elodie Marchadier, Mark Hoebeke, Stéphane Aymerich, Dörte Becher, Paola Bisicchia, Eric Botella, Olivier Delumeau, Geoff Doherty, Emma L Denham, Mark J Fogg, Vincent Fromion, Anne Goelzer, Annette Hansen, Elisabeth Härtig, Colin R Harwood, Georg Homuth, Hanne Jarmer, Matthieu Jules, Edda Klipp, Ludovic Le Chat, François Lecointe, Peter Lewis, Wolfram Liebermeister, Anika March, Ruben A T Mars, Priyanka Nannapaneni, David Noone, Susanne Pohl, Bernd Rinn, Frank Rügheimer, Praveen K Sappa, Franck Samson, Marc Schaffer, Benno Schwikowski, Leif Steil, Jörg Stülke, Thomas Wiegert, Kevin M Devine, Anthony J Wilkinson, Jan Maarten van Dijl, Michael Hecker, Uwe Völker, Philippe Bessières, Philippe Noirot
Condition-dependent transcriptome reveals high-level regulatory architecture in Bacillus subtilis.
Science: 2012, 335(6072);1103-6
[PubMed:22383849] [WorldCat.org] [DOI] (I p)