PrpE

• Description: protein tyrosine phosphatase
  
  

Gene name	prpE
Synonyms	yjbP
Essential	no
Product	protein tyrosine phosphatase
Function	unknown
Gene expression levels in SubtiExpress: prpE
MW, pI	27 kDa, 6.747
Gene length, protein length	732 bp, 244 aa
Immediate neighbours	yjbO, yjbQ
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

protein modification

The gene

Basic information

• Locus tag: BSU11630

Phenotypes of a mutant

• • increased amounts of small aci-soluble spore proteins (SASPs) PubMed

Database entries

• BsubCyc: BSU11630

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: P₁,P(4)-bis(5'-guanosyl) tetraphosphate + H₂O = GTP + GMP (according to Swiss-Prot)

• Protein family: prpE family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: forespore (according to Swiss-Prot)

Database entries

• BsubCyc: BSU11630

• Structure:

• UniProt: O31614

• KEGG entry: [2]

• E.C. number: 3.6.1.17

Expression and regulation

• Operon: prpE PubMed

• Expression browser: prpE PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Adam Iwanicki, Krzysztof Hinc, Anna Ronowicz, Arkadiusz Piotrowski, Aleksandra Wołoszyk, Michał Obuchowski
A genome-wide transcriptional profiling of sporulating Bacillus subtilis strain lacking PrpE protein phosphatase.
Mol Genet Genomics: 2013, 288(10);469-81
[PubMed:23824080] [WorldCat.org] [DOI] (I p)

Pierre Nicolas, Ulrike Mäder, Etienne Dervyn, Tatiana Rochat, Aurélie Leduc, Nathalie Pigeonneau, Elena Bidnenko, Elodie Marchadier, Mark Hoebeke, Stéphane Aymerich, Dörte Becher, Paola Bisicchia, Eric Botella, Olivier Delumeau, Geoff Doherty, Emma L Denham, Mark J Fogg, Vincent Fromion, Anne Goelzer, Annette Hansen, Elisabeth Härtig, Colin R Harwood, Georg Homuth, Hanne Jarmer, Matthieu Jules, Edda Klipp, Ludovic Le Chat, François Lecointe, Peter Lewis, Wolfram Liebermeister, Anika March, Ruben A T Mars, Priyanka Nannapaneni, David Noone, Susanne Pohl, Bernd Rinn, Frank Rügheimer, Praveen K Sappa, Franck Samson, Marc Schaffer, Benno Schwikowski, Leif Steil, Jörg Stülke, Thomas Wiegert, Kevin M Devine, Anthony J Wilkinson, Jan Maarten van Dijl, Michael Hecker, Uwe Völker, Philippe Bessières, Philippe Noirot
Condition-dependent transcriptome reveals high-level regulatory architecture in Bacillus subtilis.
Science: 2012, 335(6072);1103-6
[PubMed:22383849] [WorldCat.org] [DOI] (I p)

Katarzyna Macur, Caterina Temporini, Gabriella Massolini, Jolanta Grzenkowicz-Wydra, Michał Obuchowski, Tomasz Bączek
Proteomic analysis of small acid soluble proteins in the spore core of Bacillus subtilis ΔprpE and 168 strains with predictions of peptides liquid chromatography retention times as an additional tool in protein identification.
Proteome Sci: 2010, 8;60
[PubMed:21092197] [WorldCat.org] [DOI] (I e)

Krzysztof Hinc, Krzysztofa Nagórska, Adam Iwanicki, Grzegorz Wegrzyn, Simone J Séror, Michal Obuchowski
Expression of genes coding for GerA and GerK spore germination receptors is dependent on the protein phosphatase PrpE.
J Bacteriol: 2006, 188(12);4373-83
[PubMed:16740944] [WorldCat.org] [DOI] (P p)

Adam Iwanicki, Anna Herman-Antosiewicz, Marcin Pierechod, Simone J Séror, Michał Obuchowski
PrpE, a PPP protein phosphatase from Bacillus subtilis with unusual substrate specificity.
Biochem J: 2002, 366(Pt 3);929-36
[PubMed:12059787] [WorldCat.org] [DOI] (P p)