SpsI

• Description: glucose-1-phosphate thymidylyltransferase, spore coat polysaccharide synthesis
  
  

Gene name	spsI
Synonyms	ipa-71d
Essential	no
Product	glucose-1-phosphate thymidylyltransferase
Function	rhamnose biosynthesis, spore coat polysaccharide synthesis
Gene expression levels in SubtiExpress: spsI
MW, pI	27 kDa, 5.385
Gene length, protein length	738 bp, 246 aa
Immediate neighbours	spsJ, spsG
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

miscellaneous metabolic pathways, sporulation proteins

This gene is a member of the following regulons

SigK regulon

The gene

Basic information

• Locus tag: BSU37840

Database entries

• BsubCyc: BSU37840

• DBTBS entry: [1]

• SubtiList entry: [2]

Additional information

• A mutation was found in this gene after evolution under relaxed selection for sporulation PubMed

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • alpha-D-glucose-1-phosphate + dTTP --> dTDP-glucose PubMed

• Protein family: glucose-1-phosphate thymidylyltransferase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU37840

• Structure:

• UniProt: P39629

• KEGG entry: [3]

• E.C. number: 2.7.7.24

Expression and regulation

• Operon: spsA-spsB-spsC-spsD-spsF-spsG-spsI-spsJ-spsK-spsL PubMed

• Expression browser: spsI PubMed

• Sigma factor: SigK PubMed

• Regulation:
  • expressed late during sporulation in the mother cell (SigK) PubMed

• Regulatory mechanism:

• Additional information:
  • the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: