CotG

• Description: outer spore coat protein
  
  

Gene name	cotG
Synonyms
Essential	no
Product	spore coat protein
Function	resistance of the spore
Gene expression levels in SubtiExpress: cotG
Interactions involving this protein in SubtInteract: CotG
MW, pI	23 kDa, 10.673
Gene length, protein length	585 bp, 195 aa
Immediate neighbours	cotH, ywrF
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

sporulation proteins

This gene is a member of the following regulons

GerE regulon, GerR regulon, SigE regulon, SigK regulon

The gene

Basic information

• Locus tag: BSU36070

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • CotB-CotG PubMed

• Localization:
  • outer spore coat, localization depends on CotE PubMed

Database entries

• Structure:

• UniProt: P39801

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: cotG PubMed

• Expression browser: cotG PubMed

• Sigma factor: SigE PubMed, SigK PubMed

• Regulation:
  • expressed late during sporulation in the mother cell (SigE, SigK, GerE) PubMed

• Regulatory mechanism:
  • GerE: transcription activation PubMed
  • GerR: transcription activation PubMed

• Additional information: the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Peter T McKenney, Patrick Eichenberger
Dynamics of spore coat morphogenesis in Bacillus subtilis.
Mol Microbiol: 2012, 83(2);245-60
[PubMed:22171814] [WorldCat.org] [DOI] (I p)

Rosa Giglio, Renato Fani, Rachele Isticato, Maurilio De Felice, Ezio Ricca, Loredana Baccigalupi
Organization and evolution of the cotG and cotH genes of Bacillus subtilis.
J Bacteriol: 2011, 193(23);6664-73
[PubMed:21984783] [WorldCat.org] [DOI] (I p)

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

Ritsuko Kuwana, Takashi Okumura, Hiromu Takamatsu, Kazuhito Watabe
The ylbO gene product of Bacillus subtilis is involved in the coat development and lysozyme resistance of spore.
FEMS Microbiol Lett: 2005, 242(1);51-7
[PubMed:15621419] [WorldCat.org] [DOI] (P p)

Patrick Eichenberger, Masaya Fujita, Shane T Jensen, Erin M Conlon, David Z Rudner, Stephanie T Wang, Caitlin Ferguson, Koki Haga, Tsutomu Sato, Jun S Liu, Richard Losick
The program of gene transcription for a single differentiating cell type during sporulation in Bacillus subtilis.
PLoS Biol: 2004, 2(10);e328
[PubMed:15383836] [WorldCat.org] [DOI] (I p)

Rita Zilhão, Mónica Serrano, Rachele Isticato, Ezio Ricca, Charles P Moran, Adriano O Henriques
Interactions among CotB, CotG, and CotH during assembly of the Bacillus subtilis spore coat.
J Bacteriol: 2004, 186(4);1110-9
[PubMed:14762006] [WorldCat.org] [DOI] (P p)

G Hambraeus, C von Wachenfeldt, L Hederstedt
Genome-wide survey of mRNA half-lives in Bacillus subtilis identifies extremely stable mRNAs.
Mol Genet Genomics: 2003, 269(5);706-14
[PubMed:12884008] [WorldCat.org] [DOI] (P p)

A O Henriques, L R Melsen, C P Moran
Involvement of superoxide dismutase in spore coat assembly in Bacillus subtilis.
J Bacteriol: 1998, 180(9);2285-91
[PubMed:9573176] [WorldCat.org] [DOI] (P p)

M Sacco, E Ricca, R Losick, S Cutting
An additional GerE-controlled gene encoding an abundant spore coat protein from Bacillus subtilis.
J Bacteriol: 1995, 177(2);372-7
[PubMed:7814326] [WorldCat.org] [DOI] (P p)