NifZ

• Description: cysteine desulfurase, biosynthesis of 4-thiouridine in tRNA
  
  

Gene name	nifZ
Synonyms	iscSB
Essential	no
Product	cysteine desulfurase
Function	biosynthesis of 4-thiouridine in tRNA
Gene expression levels in SubtiExpress: nifZ
Interactions involving this protein in SubtInteract: NifZ
Metabolic function and regulation of this protein in SubtiPathways: Thiamin
MW, pI	41 kDa, 6.34
Gene length, protein length	1143 bp, 381 aa
Immediate neighbours	thiI, braB
Gene sequence (+200bp)	Protein sequence
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation

The gene

Basic information

• Locus tag: BSU29590

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: removes sulfuryl group from cysteine and transfers it to ThiI PubMed

• Protein family:

• Paralogous protein(s): NifS, YrvO, SufS, YcbU

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • NifZ-ThiI PubMed

• Localization:

Database entries

• Structure: 1EG5 (NifS-like protein from Thermotoga maritima, 35% identity, 55% similarity) PubMed

• UniProt: O34874

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: nifZ PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Christopher T Jurgenson, Tadhg P Begley, Steven E Ealick
The structural and biochemical foundations of thiamin biosynthesis.
Annu Rev Biochem: 2009, 78;569-603
[PubMed:19348578] [WorldCat.org] [DOI] (I p)

Original publications

Lauren J Rajakovich, John Tomlinson, Patricia C Dos Santos
Functional Analysis of Bacillus subtilis Genes Involved in the Biosynthesis of 4-Thiouridine in tRNA.
J Bacteriol: 2012, 194(18);4933-40
[PubMed:22773787] [WorldCat.org] [DOI] (I p)

J T Kaiser, T Clausen, G P Bourenkow, H D Bartunik, S Steinbacher, R Huber
Crystal structure of a NifS-like protein from Thermotoga maritima: implications for iron sulphur cluster assembly.
J Mol Biol: 2000, 297(2);451-64
[PubMed:10715213] [WorldCat.org] [DOI] (P p)