YwlE

• Description: protein arginine phosphatase
  
  

Gene name	ywlE
Synonyms	ipc-31d
Essential	no
Product	protein arginine phosphatase
Function	dephosphorylation of McsB
Gene expression levels in SubtiExpress: ywlE
MW, pI	16 kDa, 6.681
Gene length, protein length	450 bp, 150 aa
Immediate neighbours	ywlF, ywlD
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

protein modification

The gene

Basic information

• Locus tag: BSU36930

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Protein arginine phosphate + H₂O = protein arginine + phosphate PubMed

• Protein family:

• Paralogous protein(s): YfkJ

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • McsB-YwlE (dephosphorylation of McsB) PubMed

• Localization: weak oligomerization, results in inactivation of YwlE PubMed

Database entries

• Structure: 1ZGG

• UniProt: P39155

• KEGG entry: [2]

• E.C. number: 3.1.3.48

Expression and regulation

• Operon:

• Expression browser: ywlE PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • ywlE::aphA3 available from Ulf Gerth's lab
  • ywlE::tet available from Ulf Gerth's lab
  • ywlE::spec available from Ulf Gerth's lab
  • GP1458 (ywlE::aphA3), available in Stülke lab
  • GP1459 (ywlE::tet), available in Stülke lab

• Expression vector: for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Ulf Gerth's lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: available in Ulf Gerth's lab

Labs working on this gene/protein

• Ulf Gerth, Greifswald, Germany

• Fabian Commichau Göttingen, Germany