DacB

• Description: sporulation-specific penicillin-binding protein 5, D-alanyl-D-alanine carboxypeptidase
  
  

Gene name	dacB
Synonyms
Essential	no
Product	penicillin-binding protein 5, D-alanyl-D-alanine carboxypeptidase
Function	carboxypeptidase
Gene expression levels in SubtiExpress: dacB
MW, pI	42 kDa, 9.725
Gene length, protein length	1146 bp, 382 aa
Immediate neighbours	spmA, ypuI
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall synthesis, sporulation proteins, membrane proteins

This gene is a member of the following regulons

SigE regulon

The gene

Basic information

• Locus tag: BSU23190

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: modifies degree of cross-linking of glycan strands in peptidoglycan

• Protein family: peptidase S11 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cell membrane (according to Swiss-Prot)

Database entries

• Structure: 3MFD (beta-lactamase superfamily domain)

• UniProt: P35150

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: dacB-spmA-spmB PubMed

• Expression browser: dacB PubMed

• Sigma factor: SigE PubMed

• Regulation:
  • expressed early during sporulation in the mother cell (SigE) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • 1A744 ( dacB::cat), PubMed, available at BGSC
  • 1A745 ( dacB::cat), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: