SsbB

• Description: single-strand DNA-binding protein
  
  

Gene name	ssbB
Synonyms	ywpH
Essential	no
Product	single-strand DNA-binding protein
Function	required for efficient genetic transformation
Interactions involving this protein in SubtInteract: SsbB
MW, pI	12 kDa, 6.876
Gene length, protein length	339 bp, 113 aa
Immediate neighbours	glcR, ywpG
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

DNA replication, DNA repair/ recombination, phosphoproteins

This gene is a member of the following regulons

ComK regulon

The gene

Basic information

• Locus tag: BSU36310

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • binds and protects internalized foreign ssDNA PubMed

• Protein family:

• Paralogous protein(s): SsbA

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylated on Tyr by PtkA, dephosphorylated by PtpZ PubMed

• Cofactor(s):

• Effectors of protein activity: phosphorylation strongly increases DNA-binding activity PubMed

• Interactions:
  • forms tetramers PubMed
  • SsbB-ComFA PubMed
  • SsbB-CoiA PubMed
  • SsbB-DprA PubMed
  • SsbB-RecA PubMed

• Localization:
  • localizes to cell poles in competent cells PubMed
  • localizes to the DNA entry pole during transformation PubMed

Database entries

• Structure: 3VDY 3VDY (in complex with ssDNA) PubMed

• UniProt: C0SPB6

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:
  • ssbB PubMed
  • ssbB-glcR PubMed (this is unlikely according to PubMed)

• Expression browser: ssbB PubMed

• Sigma factor:

• Regulation:
  • expressed at the onset of stationary phase PubMed

• Regulatory mechanism:
  • ComK: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector: for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Tribhuwan Yadav, Begoña Carrasco, Angela R Myers, Nicholas P George, James L Keck, Juan C Alonso
Genetic recombination in Bacillus subtilis: a division of labor between two single-strand DNA-binding proteins.
Nucleic Acids Res: 2012, 40(12);5546-59
[PubMed:22373918] [WorldCat.org] [DOI] (I p)

Naomi Kramer, Jeanette Hahn, David Dubnau
Multiple interactions among the competence proteins of Bacillus subtilis.
Mol Microbiol: 2007, 65(2);454-64
[PubMed:17630974] [WorldCat.org] [DOI] (P p)

Ivan Mijakovic, Dina Petranovic, Boris Macek, Tina Cepo, Matthias Mann, Julian Davies, Peter R Jensen, Dusica Vujaklija
Bacterial single-stranded DNA-binding proteins are phosphorylated on tyrosine.
Nucleic Acids Res: 2006, 34(5);1588-96
[PubMed:16549871] [WorldCat.org] [DOI] (I e)

Jeanette Hahn, Berenike Maier, Bert Jan Haijema, Michael Sheetz, David Dubnau
Transformation proteins and DNA uptake localize to the cell poles in Bacillus subtilis.
Cell: 2005, 122(1);59-71
[PubMed:16009133] [WorldCat.org] [DOI] (P p)

Cordula Lindner, Reindert Nijland, Mariska van Hartskamp, Sierd Bron, Leendert W Hamoen, Oscar P Kuipers
Differential expression of two paralogous genes of Bacillus subtilis encoding single-stranded DNA binding protein.
J Bacteriol: 2004, 186(4);1097-105
[PubMed:14762004] [WorldCat.org] [DOI] (P p)

Mitsuo Ogura, Hirotake Yamaguchi, Kazuo Kobayashi, Naotake Ogasawara, Yasutaro Fujita, Teruo Tanaka
Whole-genome analysis of genes regulated by the Bacillus subtilis competence transcription factor ComK.
J Bacteriol: 2002, 184(9);2344-51
[PubMed:11948146] [WorldCat.org] [DOI] (P p)