AlaS

• Description: alanine-tRNA synthetase
  
  

Gene name	alaS
Synonyms
Essential	yes PubMed
Product	alanine-tRNA synthetase
Function	translation
Gene expression levels in SubtiExpress: alaS
Metabolic function and regulation of this protein in SubtiPathways: tRNA charging
MW, pI	97 kDa, 4.922
Gene length, protein length	2634 bp, 878 aa
Immediate neighbours	yrzL, yrrI
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation, essential genes

This gene is a member of the following regulons

T-box

The gene

Basic information

• Locus tag: BSU27410

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala) (according to Swiss-Prot)

• Protein family: class-II aminoacyl-tRNA synthetase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1YFS (enzyme from Aquifex aeolicus) PubMed, 3HXU (enzyme from E. coli with alanine) PubMed

• UniProt: O34526

• KEGG entry: [2]

• E.C. number: 6.1.1.7

Expression and regulation

• Operon:

• Expression browser: alaS PubMed

• Sigma factor:

• Regulation:
  • induced by alanine limitation (T-box) PubMed

• Regulatory mechanism:
  • T-box: RNA switch, transcriptional antitermination PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: